WB (Western Blot)
(Anti-MEKK1 antibody, AAA45458, Western blottingAll lanes: Anti MEKK1 (AAA45458) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 164KDObserved bind size: 164KD)
Rabbit MEKK1 Polyclonal Antibody | anti-MAP3K1 antibody
Anti-MEKK1 antibody
Gene Names
MAP3K1; MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
Reactivity
Reacts with: HumanPredicted: Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
MEKK1, Antibody; Anti-MEKK1 antibody; Mitogen-activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; M3K1_HUMAN antibody; MAP3K1 antibody; MAPK/ERK kinase kinase 1 antibody; MAPKKK1 antibody; MEK kinase 1 antibody; MEKK 1 antibodyMekk antibody; MEKK1 antibody; Mitogen activated protein kinase kinase kinase 1 antibody; Mitogen activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase antibody; Mitogen-activated protein kinase kinase kinase 1 antibodySRXY6 antibody; anti-MAP3K1 antibody
Host
Rabbit
Reactivity
Reacts with: Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
1512
Applicable Applications for anti-MAP3K1 antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1 (1418-1432aa PEVLRGQQYGRSCDV), identical to the related rat and mouse sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-MEKK1 antibody, AAA45458, Western blottingAll lanes: Anti MEKK1 (AAA45458) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 164KDObserved bind size: 164KD)
WB (Western Blot)
(Anti-MEKK1 antibody, AAA45458, Western blottingAll lanes: Anti MEKK1 (AAA45458) at 0.5ug/mlWB: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 164KDObserved bind size: 164KD)
IHC (Immunohistochemistry)
(Anti-MEKK1 antibody, AAA45458, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-MEKK1 antibody, AAA45458, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
Related Product Information for anti-MAP3K1 antibody
Description: Rabbit IgG polyclonal antibody for Mitogen-activated protein kinase kinase kinase 1(MAP3K1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
Background: MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
164,470 Da
NCBI Official Full Name
Mitogen-activated protein kinase kinase kinase 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase
NCBI Official Symbol
MAP3K1
NCBI Official Synonym Symbols
MEKK; MEKK1; SRXY6; MEKK 1; MAPKKK1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 1; MEK kinase 1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 1
UniProt Gene Name
MAP3K1
UniProt Synonym Gene Names
MAPKKK1; MEKK; MEKK1; MEK kinase 1; MEKK 1
UniProt Entry Name
M3K1_HUMAN
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Product Notes
The MAP3K1 map3k1 (Catalog #AAA45458) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MEKK1 antibody reacts with Reacts with: Human Predicted: Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MEKK1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the MAP3K1 map3k1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEKK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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