Rabbit mNeonGreen Polyclonal Antibody

mNeonGreen Rabbit pAb

Cat. Num. AAA283247

• Reactivity: Branchiostoma lanceolatum
• Applications: ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
• Purity: Affinity purification
• Synonyms: mNeonGreen, Antibody; mNeonGreen Rabbit pAb; anti-mNeonGreen antibody

• Host: Rabbit
• Reactivity: Branchiostoma lanceolatum
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
• Sequence: MGRDLYDDDDKDPLEMVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDGASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIISTFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHS

• Applicable Applications for anti-mNeonGreen antibody: ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
• Cross Reactivity: Species independent
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 10-236 of Branchiostoma lanceolatum mNeonGreen.
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of 293F-6Xhis-mNeonGreen-S-tag(C) and 293F cells using mNeonGreen Rabbit pAb(AAA283247) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of lysates from wild type (WT) and 293T cells transfected with mNeonGreen using mNeonGreen Rabbit pAb (AAA283247) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 0.5s.)

Related Product Information for anti-mNeonGreen antibody

Mutations were performed on LanYFP, and mNeonGreen (GenBank login number KC295282) was identified. In addition to the additional EGFP type terminal 9, a total of 21 mutations were found relative to the tetramer LanYFP (F15I, R25Q, A45D, Q56H, F67Y, K79V, S100V, F115A, I118K, V140R, T141S, M143K, L144T, D156K, T158S, S163N, Q168R, V171A, N174T, I185Y, F192Y). It is a yellow green fluorescent protein derived from amphioxus. As a fusion protein, mNeonGreen is a very useful tool for reporting gene expression, tracking cell lines, and determining subcellular protein localization.On the protein sequence level, only 20-25% of the mNeonGreen sequences are consistent with ordinary GFP derivative sequences; MNeonGreen has a maximum excitation value at 506 nm and a maximum emission value at 517 nm (Shaner et al., 2013). MNeonGreen is compatible with most GFP filter sets. Obviously, when using a GFP filter, it is three times brighter than GFP; The optimization of filters can further increase their brightness, In addition, mNeonGreen appears to be more stable than EGFP and less sensitive to laser induced bleaching. Therefore, mNeonGreen is particularly suitable for confocal microscopy and super-resolution microscopy, especially when studying low-level expression of fusion proteins.

Product Categories/Family for anti-mNeonGreen antibody

Tagged protein; Antibodies