Porcine Probable ATP-dependent RNA helicase DHX58 (DHX58) ELISA Kit | DHX58 elisa kit

Porcine Probable ATP-dependent RNA helicase DHX58 (DHX58) ELISA Kit

Cat. Num. AAA27287

• Gene Names: DHX58; LGP2; RLR-3; D11LGP2; D11lgp2e
• Reactivity: Porcine
• Synonyms: Probable ATP-dependent RNA helicase DHX58 (DHX58); Porcine Probable ATP-dependent RNA helicase DHX58 (DHX58) ELISA Kit; DHX58 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of DHX58. No significant cross-reactivity or interference between DHX58 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DHX58 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for DHX58 elisa kit

Principle of the Assay: DHX58 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DHX58 antibody and an DHX58-HRP conjugate. The assay sample and buffer are incubated together with DHX58-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DHX58 concentration since DHX58 from samples and DHX58-HRP conjugate compete for the anti-DHX58 antibody binding site. Since the number of sites is limited, as more sites are occupied by DHX58 from the sample, fewer sites are left to bind DHX58-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DHX58 concentration in each sample is interpolated from this standard curve.

Intended Uses: This DHX58 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine DHX58. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for DHX58 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 149408122
• NCBI GeneID: 79132
• NCBI Accession #: NP_077024.2
• NCBI GenBank Nucleotide #: NM_024119.2
• Molecular Weight: 76,613 Da
• NCBI Official Full Name: probable ATP-dependent RNA helicase DHX58
• NCBI Official Synonym Full Names: DEXH (Asp-Glu-X-His) box polypeptide 58
• NCBI Official Symbol: DHX58
• NCBI Official Synonym Symbols: LGP2; RLR-3; D11LGP2; D11lgp2e
• NCBI Protein Information: probable ATP-dependent RNA helicase DHX58; RLR; RNA helicase LGP2; RIG-I-like receptor 3; protein D11Lgp2 homolog; RIG-I-like receptor LGP2; ortholog of mouse D11lgp2; probable ATP-dependent helicase LGP2
• UniProt Protein Name: Probable ATP-dependent RNA helicase DHX58
• UniProt Gene Name: DHX58
• UniProt Synonym Gene Names: D11LGP2E; LGP2; RLR-3; RLR
• UniProt Entry Name: DHX58_HUMAN