IF (Immunofluorescence)
(Staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit Mre11 Polyclonal Antibody | anti-Mre11 antibody
Phospho-Mre11 (Ser676) Antibody
Gene Names
MRE11A; ATLD; HNGS1; MRE11; MRE11B
Reactivity
Human, Mouse, Monkey
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Mre11, Antibody; Phospho-Mre11 (Ser676) Antibody; AT like disease; Ataxia telangiectasia disorder like; ATLD; DNA recombination and repair protein; Double strand break repair protein MRE11A; Double-strand break repair protein MRE11A; endo/exonuclease Mre11; HNGS1; meiotic recombination (S. cerevisiae) 11 homolog A; Meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A (S. cerevisiae); Meiotic recombination 11 homolog A; MmMRE11A; Mre 11; MRE 11a; MRE 11b; MRE11 homolog 1; MRE11 homolog A; MRE11 meiotic recombination 11 homolog A (S. cerevisiae); MRE11 meiotic recombination 11 homolog A; MRE11_HUMAN; MRE11A; MRE11b; OTTHUMP00000236830; OTTHUMP00000236831; OTTHUMP00000236832; OTTHUMP00000236833; anti-Mre11 antibody
Host
Rabbit
Reactivity
Human, Mouse, Monkey
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Mre11 (Ser676) Antibody detects endogenous levels of Mre11 only when phosphorylated at Ser676.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Mre11 antibody
ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Mre11 around the phosphorylation site of Ser676.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Ubiquitinated following DNA damage. Ubiquitination triggers interaction with UBQLN4, leading to MRE11 removal from chromatin and degradation by the proteasome.
Subunit Structure
Component of the MRN complex composed of two heterodimers RAD50/MRE11 associated with a single NBN. As part of the MRN complex, interacts with MCM9; the interaction recruits the complex to DNA repair sites. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11 and NBN. Found in a complex with TERF2. Interacts with DCLRE1C/Artemis and DCLRE1B/Apollo. Interacts with ATF2. Interacts with EXD2. Interacts with MRNIP. Interacts with SAMHD1; leading to stimulate 3'-5' exonuclease activity. Interacts (when ubiquitinated) with UBQLN4 (via its UBA domain). (Microbial infection) Interacts with herpes simplex virus 1 protein UL12.
Similarity
Belongs to the MRE11/RAD32 family.
Subcellular Location
Nucleus. Chromosome>Telomere. Chromosome.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.
Note: Localizes to discrete nuclear foci after treatment with genotoxic agents.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(Staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence)
(Staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of Mre11 (Phospho-Ser676) using serum treated COS7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of Mre11 (Phospho-Ser676) using serum treated COS7 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-Mre11 antibody
Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11 to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
80,593 Da
NCBI Official Full Name
double-strand break repair protein MRE11A isoform 2
NCBI Official Synonym Full Names
MRE11 meiotic recombination 11 homolog A (S. cerevisiae)
NCBI Official Symbol
MRE11A
NCBI Official Synonym Symbols
ATLD; HNGS1; MRE11; MRE11B
NCBI Protein Information
double-strand break repair protein MRE11A; AT-like disease; MRE11 homolog 1; MRE11 homolog A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A; DNA recombination and repair protein
UniProt Protein Name
Double-strand break repair protein MRE11A
UniProt Gene Name
MRE11A
UniProt Synonym Gene Names
HNGS1; MRE11; MRE11 homolog 1
UniProt Entry Name
MRE11_HUMAN
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Product Notes
The Mre11 mre11a (Catalog #AAA329917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Mre11 (Ser676) Antibody reacts with Human, Mouse, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Mre11 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Mre11 mre11a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mre11, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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