IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MSH2 antibody (AAA282388). Western blot was performed from the immunoprecipitate using MSH2 antibody (AAA282388) at a dilution of 1:3000.)
Rabbit anti-Human, Rat MSH2 Polyclonal Antibody | anti-MSH2 antibody
[KO Validated] MSH2 Rabbit pAb
Gene Names
MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
Reactivity
Human, Rat
Applications
Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity purification
Synonyms
MSH2, Antibody; [KO Validated] MSH2 Rabbit pAb; FCC1; COCA1; HNPCC; LCFS2; MSH-2; hMSH2; HNPCC1; LYNCH1; MMRCS2; anti-MSH2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
MAVQPKETLQLESAAEVGFVRFFQGMPEKPTTTVRLFDRGDFYTAHGEDALLAAREVFKTQGVIKYMGPAGAKNLQSVVLSKMNFESFVKDLLLVRQYRVEVYKNRAGNKASKENDWYLAYKASPGNLSQFEDILFGNNDMSASIGVVGVKMSAVDGQRQVGVGYVDSIQRKLGLCEFPDNDQFSNLEALLIQIGPKECVLPGGETAGDMGKLRQIIQRGGILITERKKADFSTKDIYQDLNRLLKGKKGEQMNSAVLPEMENQVAVSSLSAVIKFLELLSDDSNFGQFELTTFDFSQYM
Applicable Applications for anti-MSH2 antibody
IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Positive Samples
HeLa, A-549
Cellular Location
Nucleus
Research Area
Epigenetics Nuclear Signaling, DNA Damage Repair, Cancer, Tumor suppressors, Cell Biology Developmental Biology, Apoptosis
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Ship: Ice Pack
Ship: Ice Pack
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MSH2 antibody (AAA282388). Western blot was performed from the immunoprecipitate using MSH2 antibody (AAA282388) at a dilution of 1:3000.)
IP (Immunoprecipitation)
(Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug MSH2 antibody (AAA282388). Western blot was performed from the immunoprecipitate using MSH2 antibody (AAA282388) at a dilution of 1:3000.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and MSH2 Rabbit pAb knockout (KO) HeLa cells, using MSH2 Rabbit pAb antibody (AAA282388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of extracts from wild type(WT) and MSH2 Rabbit pAb knockout (KO) HeLa cells, using MSH2 Rabbit pAb antibody (AAA282388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of A-549, using MSH2 Rabbit pAb antibody (AAA282388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
WB (Western Blot)
(Western blot analysis of A-549, using MSH2 Rabbit pAb antibody (AAA282388) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
Related Product Information for anti-MSH2 antibody
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-MSH2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
934
NCBI Official Full Name
DNA mismatch repair protein Msh2
NCBI Official Synonym Full Names
mutS homolog 2
NCBI Official Symbol
MSH2
NCBI Official Synonym Symbols
FCC1; COCA1; HNPCC; LCFS2; HNPCC1
NCBI Protein Information
DNA mismatch repair protein Msh2; hMSH2; mutS homolog 2, colon cancer, nonpolyposis type 1
UniProt Protein Name
DNA mismatch repair protein Msh2
UniProt Gene Name
MSH2
UniProt Synonym Gene Names
hMSH2
UniProt Entry Name
MSH2_HUMAN
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Product Notes
The MSH2 msh2 (Catalog #AAA282388) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The [KO Validated] MSH2 Rabbit pAb reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MSH2 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the MSH2 msh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MAVQPKETLQ LESAAEVGFV RFFQGMPEKP TTTVRLFDRG DFYTAHGEDA LLAAREVFKT QGVIKYMGPA GAKNLQSVVL SKMNFESFVK DLLLVRQYRV EVYKNRAGNK ASKENDWYLA YKASPGNLSQ FEDILFGNND MSASIGVVGV KMSAVDGQRQ VGVGYVDSIQ RKLGLCEFPD NDQFSNLEAL LIQIGPKECV LPGGETAGDM GKLRQIIQRG GILITERKKA DFSTKDIYQD LNRLLKGKKG EQMNSAVLPE MENQVAVSSL SAVIKFLELL SDDSNFGQFE LTTFDFSQYM. It is sometimes possible for the material contained within the vial of "MSH2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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