Rabbit anti-Human MSH2 Polyclonal Antibody | anti-MSH2 antibody

MSH2

Cat. Num. AAA297732

• Gene Names: MSH2; FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• Reactivity: Human
• Applications: Western Blot
• Synonyms: MSH2, Antibody; MSH2; DNA mismatch repair protein Msh2; hMSH2; MutS protein homolog 2; Purified Rabbit Polyclonal Antibody (Pab); anti-MSH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between amino acids from human.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

• Applicable Applications for anti-MSH2 antibody: WB (Western Blot)
• Antigen Source: Human
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(WB: 1:1000; All lanes: Anti-MSH2 antibody at 1:1000 dilutionLane 1: HepG2 whole cell lysateLane 2: Hela whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) E42at 1/15000 dilution.Observed band size: 105KDaBlocking/Dilution buffer: 5% NFDM/TBST.)

WB (Western Blot)

(WB: 1:1000; All lanes: Anti-MSH2 antibody at 1:1000 dilutionLane 1: HepG2 whole cell lysateLane 2: SW480 whole cell lysateLane 3: Hela whole cell lysateLane 4: HCT116 whole cell lysateLysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) E42at 1/15000 dilution.Observed band size: 105KDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-MSH2 antibody

Component of the post-replicative DNA mismatch repair system "MMR". Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. Recruits DNA helicase MCM9 to chromatin which unwinds the mismatch containing DNA strand (PubMed:26300262). ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.

Product Categories/Family for anti-MSH2 antibody

Primary Antibodies; Antibody Collections; Cancer; Liver negative; Endometrium negative; Pancreas negative

References

Fishel R.,et al.Cell 75:1027-1038(1993).Fishel R.,et al.Cell 77:167-167(1994).Leach F.S.,et al.Cell 75:1215-1225(1993).Kolodner R.D.,et al.Genomics 24:516-526(1994).Wijnen J.,et al.Am. J. Hum. Genet. 56:1060-1066(1995).

NCBI and Uniprot Product Information

• NCBI GI #: 1171032
• NCBI GeneID: 4436
• UniProt Accession #: P43246; O75488; B4E2Z2
• Molecular Weight: 934
• NCBI Official Full Name: DNA mismatch repair protein Msh2
• NCBI Official Synonym Full Names: mutS homolog 2
• NCBI Official Symbol: MSH2
• NCBI Official Synonym Symbols: FCC1; COCA1; HNPCC; LCFS2; HNPCC1
• NCBI Protein Information: DNA mismatch repair protein Msh2; hMSH2; mutS homolog 2, colon cancer, nonpolyposis type 1
• UniProt Protein Name: DNA mismatch repair protein Msh2
• UniProt Gene Name: MSH2
• UniProt Synonym Gene Names: hMSH2
• UniProt Entry Name: MSH2_HUMAN