IF (Immunofluorescence)
(Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
Rabbit Natriuretic Receptor C / PGCC Polyclonal Antibody | anti-PGCC antibody
Rabbit Polyclonal PGCC antibody
Gene Names
Gucy2c; GCC
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Synonyms
Natriuretic Receptor C / PGCC, Antibody; Rabbit Polyclonal PGCC antibody; ANPRC antibody, Atrial natriuretic peptide C type receptor antibody, C5orf23 antibody, FLJ14054 antibody, GUCY2B antibody, MGC22189 antibody, Natriuretic peptide receptor C/iguanylate cyclase C (atrionatriuretic peptide receptor C) antibody, Npr3 antibody, NPRC antibody.; anti-PGCC antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Form/Format
Affinity Purified Immunoglobulins
Concentration
0.65 mg/ml IgG in antibody stabilization buffer (varies by lot)
Sequence Length
1072
Applicable Applications for anti-PGCC antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, CM (Confocal Microscopy)
Immunogen
Synthetic C-terminal peptide corresponding to unique amino acid sequences on Particulate Guanylyl Cyclase C protein.
Molecular Function
Lyase, Receptor
Structure
Homotrimer. Interacts via its C-terminal region with PDZK2. Interacts with the lectin chaperone VIP36
Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endoplasmic Reticulum membrane
Preparation and Storage
-20 degree C for long term storage
IF (Immunofluorescence)
(Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
IF (Immunofluorescence)
(Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample.)
WB (Western Blot)
WB (Western Blot)
Related Product Information for anti-PGCC antibody
Particulate Guanylate Cyclase C Antibody
The intracellular levels of Cyclic GMP (cGMP) are maintained by the activity of opposing enzymes: synthesizing gualylyl cyciases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate). While they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds nitric oxide that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. There is significant structural homology among various PGCs: there is a large N-terminal extracellular domain(ECD), a single transmembrane domain, a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase C (PGC-C), a member of membrane-bound guanylyl cyclases, is a receptor protein for guanylin and uroguanylin. The binding of a ligand to the extracellular domain of PGC-C triggers signal transduction, resulting in the regulation of intestinal fluids and electrolytes (1). A previous study proposed that a ligand-binding site on PGC-C is localized near the transmembrane region. The PGC-C type guanylate cyclase is a receptor for heat stable enterotoxin. PGC-C protein contains an extra-cellular amino acid sequence that is divergent from other PGCs, but retains several structural motifs (KLD, ECD and TMD). Disrupted activity of PGC-C may be involved in acute diarrhea.
The Anti-PGC-C-selective antibodies were generated against conserved sequences near the C-terminal end of the protein that are unique to PGC-C protein. The PGC-C-selective antibodies are affinity purified against immobilized antigen based affinity chromatography that yielded epitope-specific antibodies. Antigenic blocking peptides (P-PGCC) and westem blot positive control (PC-PGCC) in rearly to use SDS-sample buffer are available. The PGC-C antibodies label a 127-130 kDa protein in various tissues including intestine kidney and spleen. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.
The intracellular levels of Cyclic GMP (cGMP) are maintained by the activity of opposing enzymes: synthesizing gualylyl cyciases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate). While they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, soluble GC (sGC) contains a heme group and binds nitric oxide that activates the enzyme, while particulate GC (PGCs) are stimulated by natriuretic peptides. Particulate forms of guanylyl cyclases have been shown to function as natriuretic peptide receptors. In response to G-protein coupled receptor stimulation, the cGMP can be produced from GTP by either soluble guanylate cyclase (sGC), or by PGC. The sGC are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. There is significant structural homology among various PGCs: there is a large N-terminal extracellular domain(ECD), a single transmembrane domain, a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Guanylyl cyclase C (PGC-C), a member of membrane-bound guanylyl cyclases, is a receptor protein for guanylin and uroguanylin. The binding of a ligand to the extracellular domain of PGC-C triggers signal transduction, resulting in the regulation of intestinal fluids and electrolytes (1). A previous study proposed that a ligand-binding site on PGC-C is localized near the transmembrane region. The PGC-C type guanylate cyclase is a receptor for heat stable enterotoxin. PGC-C protein contains an extra-cellular amino acid sequence that is divergent from other PGCs, but retains several structural motifs (KLD, ECD and TMD). Disrupted activity of PGC-C may be involved in acute diarrhea.
The Anti-PGC-C-selective antibodies were generated against conserved sequences near the C-terminal end of the protein that are unique to PGC-C protein. The PGC-C-selective antibodies are affinity purified against immobilized antigen based affinity chromatography that yielded epitope-specific antibodies. Antigenic blocking peptides (P-PGCC) and westem blot positive control (PC-PGCC) in rearly to use SDS-sample buffer are available. The PGC-C antibodies label a 127-130 kDa protein in various tissues including intestine kidney and spleen. Antibodies can be conjugated to fluorophores and other secondary enzymes upon request at nominal cost.
Product Categories/Family for anti-PGCC antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
123,467 Da
NCBI Official Full Name
guanylyl cyclase
NCBI Official Synonym Full Names
guanylate cyclase 2C
NCBI Official Symbol
Gucy2c
NCBI Official Synonym Symbols
GCC
NCBI Protein Information
heat-stable enterotoxin receptor
UniProt Protein Name
Heat-stable enterotoxin receptor
UniProt Gene Name
Gucy2c
UniProt Synonym Gene Names
Guc2c; STA receptor; GC-C
UniProt Entry Name
GUC2C_RAT
Similar Products
Product Notes
The PGCC gucy2c (Catalog #AAA75655) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal PGCC antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Natriuretic Receptor C / PGCC can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), ELISA, CM (Confocal Microscopy). Researchers should empirically determine the suitability of the PGCC gucy2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Natriuretic Receptor C / PGCC, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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