WB (Western Blot)
(Detection of human NBS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NBS1 antibody AAA211396 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
Rabbit anti-Human NBS1 Polyclonal Antibody | anti-NBS1 antibody
Rabbit anti-NBS1 Antibody, Affinity Purified
Gene Names
NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NBS1, Antibody; Rabbit anti-NBS1 Antibody, Affinity Purified; NBS; P95; Nijmegen breakage syndrome protein 1; Nijmegen breakage syndrome 1 (nibrin); NBS1; p95 protein of the MRE11/RAD50 complex; cell cycle regulatory protein p95; AT-V2; AT-V1; ATV; nibrin; Nibrin; anti-NBS1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NBS1 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Orangutan
Immunogen
Between 704 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human NBS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NBS1 antibody AAA211396 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human NBS1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NBS1 antibody AAA211396 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibodies and which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human NBS1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NBS1 (Cat. No. AAA211396 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human NBS1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-NBS1 (Cat. No. AAA211396 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-NBS1 antibody
Mutations in NBS1 (Nijmegen Breakage Syndrome 1), also known as nibrin, are associated with the autosomal recessive syndrome, Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. At the cellular level, NBS1 is part of the MRE11/RAD50 double-strand break repair complex that is critical to the DNA damage response, DNA recombination, telomere integrity, and cell cycle checkpoint control.
Product Categories/Family for anti-NBS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,959 Da
NCBI Official Full Name
nibrin
NCBI Official Synonym Full Names
nibrin
NCBI Official Symbol
NBN
NCBI Official Synonym Symbols
ATV; NBS; P95; NBS1; AT-V1; AT-V2
NCBI Protein Information
nibrin; cell cycle regulatory protein p95; Nijmegen breakage syndrome 1 (nibrin); p95 protein of the MRE11/RAD50 complex
UniProt Protein Name
Nibrin
UniProt Gene Name
NBN
UniProt Synonym Gene Names
NBS; NBS1; P95
UniProt Entry Name
NBN_HUMAN
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Product Notes
The NBS1 nbn (Catalog #AAA211396) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NBS1 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NBS1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NBS1 nbn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NBS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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