WB (Western Blot)
(Detection of mouse NBS1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NBS1 antibody AAA211394 (lot AAA211394-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Mouse NBS1 Polyclonal Antibody | anti-NBS1 antibody
Rabbit anti-NBS1 Antibody, Affinity Purified
Gene Names
Nbn; Nbs1
Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
NBS1, Antibody; Rabbit anti-NBS1 Antibody, Affinity Purified; nibrin; Nbs1; cell cycle regulatory protein p95; nijmegen breakage syndrome protein 1 homolog; Nibrin; anti-NBS1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-NBS1 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 701 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of mouse NBS1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NBS1 antibody AAA211394 (lot AAA211394-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of mouse NBS1 by western blot. Samples: Whole cell lysate (50 ug) from L1210 and CT26.WT cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NBS1 antibody AAA211394 (lot AAA211394-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of mouse NBS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NBS1 antibody AAA211394 (lot AAA211394-2) used for IP at 3 ug per reaction. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibody BL5225. For blotting immunoprecipitated NBS1, AAA211394 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of mouse NBS1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from L1210 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NBS1 antibody AAA211394 (lot AAA211394-2) used for IP at 3 ug per reaction. NBS1 was also immunoprecipitated by rabbit anti-NBS1 antibody BL5225. For blotting immunoprecipitated NBS1, AAA211394 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-NBS1 antibody
Mutations in NBS1 (Nijmegen Breakage Syndrome 1), also known as nibrin, are associated with the autosomal recessive syndrome, Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. At the cellular level, NBS1 is part of the MRE11/RAD50 double-strand break repair complex that is critical to the DNA damage response, DNA recombination, telomere integrity, and cell cycle checkpoint control.
Product Categories/Family for anti-NBS1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
83,795 Da
NCBI Official Full Name
nibrin
NCBI Official Synonym Full Names
nibrin
NCBI Official Symbol
Nbn
NCBI Official Synonym Symbols
Nbs1
NCBI Protein Information
nibrin
UniProt Protein Name
Nibrin
UniProt Gene Name
Nbn
UniProt Synonym Gene Names
Nbs1
UniProt Entry Name
NBN_MOUSE
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Product Notes
The NBS1 nbn (Catalog #AAA211394) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-NBS1 Antibody, Affinity Purified reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NBS1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the NBS1 nbn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NBS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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