FCM/FACS (Flow Cytometry)
(NKX2-1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit NKX2-1 Polyclonal Antibody | anti-NKX2-1 antibody
NKX2-1 Antibody (N-term)
Gene Names
NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NKX2-1, Antibody; NKX2-1 Antibody (N-term); Homeobox protein Nkx-21; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; TTF-1; Thyroid-specific enhancer-binding protein; T/EBP; NKX2-1; NKX2A; TITF1; TTF1; anti-NKX2-1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
2-1
Sequence Length
401
Applicable Applications for anti-NKX2-1 antibody
FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(NKX2-1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(NKX2-1 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
WB (Western Blot)
(NKX2-1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NKX2-1 antibody detected the NKX2-1 protein (arrow).)
WB (Western Blot)
(NKX2-1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NKX2-1 antibody detected the NKX2-1 protein (arrow).)
Related Product Information for anti-NKX2-1 antibody
This gene encodes a protein initially identified as a
thyroid-specific transcription factor. The encoded protein binds to
thyroglobulin promoter and regulates the expression of
thyroid-specific genes but has also been shown to regulate the
expression of genes involved in morphogenesis. Mutations and
deletions in this gene are associated with benign hereditary
chorea, choreoathetosis, congenital hypothyroidism, and neonatal
respiratory distress, and may be associated with thyroid cancer.
Multiple transcript variants encoding different isoforms have been
found for this gene.
thyroid-specific transcription factor. The encoded protein binds to
thyroglobulin promoter and regulates the expression of
thyroid-specific genes but has also been shown to regulate the
expression of genes involved in morphogenesis. Mutations and
deletions in this gene are associated with benign hereditary
chorea, choreoathetosis, congenital hypothyroidism, and neonatal
respiratory distress, and may be associated with thyroid cancer.
Multiple transcript variants encoding different isoforms have been
found for this gene.
References
Kim, J.H., et al. Acta Cytol. 54(3):277-282(2010)
Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010)
Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010)
Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010) :
Cantara, S., et al. Thyroid Res 3 (1), 4 (2010) :
Xu, B., et al. Appl. Immunohistochem. Mol. Morphol. 18(3):244-249(2010)
Narumi, S., et al. J. Clin. Endocrinol. Metab. 95(4):1981-1985(2010)
Guillot, L., et al. Hum. Mutat. 31 (2), E1146-E1162 (2010) :
Cantara, S., et al. Thyroid Res 3 (1), 4 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38596
NCBI Official Full Name
homeobox protein Nkx-2.1 isoform 1
NCBI Official Synonym Full Names
NK2 homeobox 1
NCBI Official Symbol
NKX2-1
NCBI Official Synonym Symbols
BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1
NCBI Protein Information
homeobox protein Nkx-2.1
UniProt Protein Name
Homeobox protein Nkx-2.1
UniProt Gene Name
NKX2-1
UniProt Synonym Gene Names
TTF-1; T/EBP
UniProt Entry Name
NKX21_HUMAN
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Product Notes
The NKX2-1 nkx2-1 (Catalog #AAA290041) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 2-1. The NKX2-1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NKX2-1 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NKX2-1 nkx2-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NKX2-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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