IHC (Immunohiostchemistry)
(NONO Antibody (N-term) (AAA283693)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NONO Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit NONO Polyclonal Antibody | anti-NONO antibody
NONO Antibody (N-term)
Gene Names
NONO; P54; NMT55; NRB54; P54NRB; PPP1R114
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
NONO, Antibody; NONO Antibody (N-term); Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex; 52 kDa subunit; NMT55; p54(nrb); p54nrb; NONO; NRB54; anti-NONO antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This NONO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human NONO.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.46 (varies by lot)
Sequence Positions
45-74
Sequence Length
471
Applicable Applications for anti-NONO antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(NONO Antibody (N-term) (AAA283693)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NONO Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(NONO Antibody (N-term) (AAA283693)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NONO Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(NONO Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NONO antibody detected the NONO protein (arrow).)
WB (Western Blot)
(NONO Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the NONO antibody detected the NONO protein (arrow).)
Related Product Information for anti-NONO antibody
This gene encodes an RNA-binding protein which plays
various roles in the nucleus, including transcriptional regulation
and RNA splicing. A rearrangement between this gene and the
transcription factor E3 gene has been observed in papillary renal
cell carcinoma. Alternatively spliced transcript variants have been
described. Pseudogenes exist on Chromosomes 2 and 16. [provided by
RefSeq].
various roles in the nucleus, including transcriptional regulation
and RNA splicing. A rearrangement between this gene and the
transcription factor E3 gene has been observed in papillary renal
cell carcinoma. Alternatively spliced transcript variants have been
described. Pseudogenes exist on Chromosomes 2 and 16. [provided by
RefSeq].
References
Marko, M., et al. Exp. Cell Res. 316(3):390-400(2010)
Dong, X., et al. Mol. Endocrinol. 23(8):1147-1160(2009)
Sikora, D., et al. Virology 390(1):71-78(2009)
Clemson, C.M., et al. Mol. Cell 33(6):717-726(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
Dong, X., et al. Mol. Endocrinol. 23(8):1147-1160(2009)
Sikora, D., et al. Virology 390(1):71-78(2009)
Clemson, C.M., et al. Mol. Cell 33(6):717-726(2009)
Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54232
NCBI Official Full Name
non-POU domain-containing octamer-binding protein isoform 1
NCBI Official Synonym Full Names
non-POU domain containing, octamer-binding
NCBI Official Symbol
NONO
NCBI Official Synonym Symbols
P54; NMT55; NRB54; P54NRB; PPP1R114
NCBI Protein Information
non-POU domain-containing octamer-binding protein
UniProt Protein Name
Non-POU domain-containing octamer-binding protein
UniProt Gene Name
NONO
UniProt Synonym Gene Names
NRB54; NonO protein; p54nrb
UniProt Entry Name
NONO_HUMAN
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Product Notes
The NONO nono (Catalog #AAA283693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 45-74. The NONO Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's NONO can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the NONO nono for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NONO, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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