Application Data
((0.001ug/ml) staining in A431(A), HeLa(B), MCF7(C), HepG2(D) and A549(E) cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Mouse Pan Cytokeratin Monoclonal Antibody | anti-KRT6C antibody
Pan Cytokeratin Monoclonal Antibody [C-11]
Gene Names
KRT6C; K6E; KRT6E; PPKNEFD
Reactivity
Mouse, Rat, Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A purified
Synonyms
Pan Cytokeratin, Antibody; Pan Cytokeratin Monoclonal Antibody [C-11]; anti-KRT6C antibody
Host
Mouse
Reactivity
Mouse, Rat, Human
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
C11
Purity/Purification
Protein A purified
Form/Format
Purified antibody (from supernatant) containing PBS + 0.1% sodium azide
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT6C antibody
FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Cytoskeletal preparation from A431 cells
Target Antigen
Pan Cytokeratin
Pubmed ID
32909189, 33536206, 33542207, 33562096, 33667891, 33706249, 33907276, 33946667, 33044631
Application Data
((0.001ug/ml) staining in A431(A), HeLa(B), MCF7(C), HepG2(D) and A549(E) cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Application Data
((0.001ug/ml) staining in A431(A), HeLa(B), MCF7(C), HepG2(D) and A549(E) cells lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
FCM/FACS (Flow Cytometry)
(Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)
FCM/FACS (Flow Cytometry)
(Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Fluorescein Isotype control)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
IF (Immunofluorescence)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells. Primary incubation 1hr (1:50-1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Isotype control: Anti-Fluorescein followed by Alexa Fluor 488 secondary antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60 kDa
NCBI Official Full Name
keratin, type II cytoskeletal 6C
NCBI Official Synonym Full Names
keratin 6C, type II
NCBI Official Symbol
KRT6C
NCBI Official Synonym Symbols
K6E; KRT6E; PPKNEFD
NCBI Protein Information
keratin, type II cytoskeletal 6C; CK-6C; CK-6E; K6C; cytokeratin-6C; cytokeratin-6E; keratin 6E; keratin K6h; type-II keratin Kb12
UniProt Protein Name
Keratin, type II cytoskeletal 6C
UniProt Gene Name
KRT6C
UniProt Synonym Gene Names
KRT6E; CK-6C; CK-6E; K6C
UniProt Entry Name
K2C6C_HUMAN
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Product Notes
The KRT6C krt6c (Catalog #AAA59578) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Pan Cytokeratin Monoclonal Antibody [C-11] reacts with Mouse, Rat, Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pan Cytokeratin can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT6C krt6c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pan Cytokeratin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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