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FCM/FACS (Flow Cytometry)
(PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human PCDH20 Polyclonal Antibody | anti-PCDH20 antibody
PCDH20 Antibody (Center)
Gene Names
PCDH20; PCDH13
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
PCDH20, Antibody; PCDH20 Antibody (Center); Protocadherin-20; Protocadherin-13; PCDH20; PCDH13; anti-PCDH20 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This PCDH20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-472 amino acids from the Central region of human PCDH20.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
445-472
Sequence Length
951
Applicable Applications for anti-PCDH20 antibody
FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(PCDH20 Antibody (Center) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PCDH20 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of PCDH20 Antibody (Center) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IHC (Immunohiostchemistry)
(PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(PCDH20 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PCDH20 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(PCDH20 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCDH20 antibody detected the PCDH20 protein (arrow).)
WB (Western Blot)
(PCDH20 Antibody (Center) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the PCDH20 antibody detected the PCDH20 protein (arrow).)
Related Product Information for anti-PCDH20 antibody
This gene belongs to the protocadherin gene family, a
subfamily of the cadherin superfamily. This gene encodes a protein
which contains 6 extracellular cadherin domains, a transmembrane
domain and a cytoplasmic tail differing from those of the classical
cadherins. Although its specific function is undetermined, the
cadherin-related neuronal receptor is thought to play a role in the
establishment and function of specific cell-cell connections in the
brain.
subfamily of the cadherin superfamily. This gene encodes a protein
which contains 6 extracellular cadherin domains, a transmembrane
domain and a cytoplasmic tail differing from those of the classical
cadherins. Although its specific function is undetermined, the
cadherin-related neuronal receptor is thought to play a role in the
establishment and function of specific cell-cell connections in the
brain.
Product Categories/Family for anti-PCDH20 antibody
References
Suzuki, S.T. Exp. Cell Res. 261(1):13-18(2000)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Cross, S.H., et al. Nat. Genet. 6(3):236-244(1994)
Nollet, F., et al. J. Mol. Biol. 299(3):551-572(2000)
Yagi, T., et al. Genes Dev. 14(10):1169-1180(2000)
Wu, Q., et al. Proc. Natl. Acad. Sci. U.S.A. 97(7):3124-3129(2000)
Cross, S.H., et al. Nat. Genet. 6(3):236-244(1994)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104919
NCBI Official Full Name
protocadherin-20
NCBI Official Synonym Full Names
protocadherin 20
NCBI Official Symbol
PCDH20
NCBI Official Synonym Symbols
PCDH13
NCBI Protein Information
protocadherin-20
UniProt Protein Name
Protocadherin-20
UniProt Gene Name
PCDH20
UniProt Synonym Gene Names
PCDH13
UniProt Entry Name
PCD20_HUMAN
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Product Notes
The PCDH20 pcdh20 (Catalog #AAA286428) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 445-472. The PCDH20 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PCDH20 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the PCDH20 pcdh20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PCDH20, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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