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IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
Rabbit PEX3 Polyclonal Antibody | anti-PEX3 antibody
PEX3 (IN) Antibody
Gene Names
PEX3; TRG18; PBD10A
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunofluorescence
Purity
PEX3 Antibody is affinity chromatography purified via peptide column.
Synonyms
PEX3; Polyclonal Antibody; PEX3 (IN) Antibody; PEX3 Antibody: Peroxisomal biogenesis factor 3; Peroxin-3; Peroxisomal assembly protein PEX3; anti-PEX3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
PEX3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; PEX3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1mg/mL (varies by lot)
Applicable Applications for anti-PEX3 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 2ug/mL; IF: 20ug/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested.
Immunogen
Anti-PEX3 antibody (9667) was raised against a peptide corresponding to 14 amino acids near the center of human PEX3. The immunogen is located within the center of PEX3.
Homology
Predicted species reactivity based on immunogen sequence: monkey (100%), cow (100%), chicken (100%)
Research Area
Signal Transduction
NCBI Organism
Homo sapiens
Isoforms
Human PEX3 has 1 isoform, (373aa, 42kD). Mouse PEX3 has 1 isoform (372aa, 42kD). Rat PEX3 has one isoform (372aa, 42kD). can detect PEX3 in human, mouse and rat.
Preparation and Storage
PEX3 antibody can be stored at 4 degree C up to one year. Antibodies should not be exposed to prolonged high temperatures.
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
IF (Immunofluorescence)
(Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with AAA11048 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)
WB (Western Blot)
(Figure 3 Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 3 Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX3 AAA11048, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate Antibodies: PEX3 AAA11048, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
WB (Western Blot)
(Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate Antibodies: PEX3 AAA11048, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)
Related Product Information for anti-PEX3 antibody
PEX3 Antibody: PEX3 is involved in peroxisome biosynthesis and integrity. It assembles membrane vesicles before the matrix proteins are translocated. As a docking factor for PEX19, PEX3 is necessary for the import of peroxisomal membrane proteins in the peroxisomes. Thus within the cell, PEX3 is stabilized by PEX19 preventing PEX3 aggregation. Mutations in the PEX19-binding domain of PEX3 reduce the affinity for PEX19 and destabilize PEX3.
Product Categories/Family for anti-PEX3 antibody
References
Ghaedi et al. Mol Biol Cell. 2000; 11(6):2085-2102
Fang et al. J Cell Biol. 2004;164(6):863-875
Hattula et al. PloS One. 2014; 9(7):e103101
Schmidt et al. Traffic. 2012; 13(9):1244-1260
Fang et al. J Cell Biol. 2004;164(6):863-875
Hattula et al. PloS One. 2014; 9(7):e103101
Schmidt et al. Traffic. 2012; 13(9):1244-1260
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,140 Da
NCBI Official Full Name
peroxisomal biogenesis factor 3
NCBI Official Synonym Full Names
peroxisomal biogenesis factor 3
NCBI Official Symbol
PEX3
NCBI Official Synonym Symbols
TRG18; PBD10A
NCBI Protein Information
peroxisomal biogenesis factor 3; peroxin-3; peroxisomal assembly protein PEX3; transformation-related protein 18
UniProt Protein Name
Peroxisomal biogenesis factor 3
UniProt Gene Name
PEX3
UniProt Entry Name
PEX3_HUMAN
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Product Notes
The PEX3 pex3 (Catalog #AAA11048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEX3 (IN) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PEX3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). WB: 2ug/mL; IF: 20ug/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the PEX3 pex3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PEX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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