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FCM (Flow Cytometry)
(Flow cytometric analysis of SiHa cells with PHF8 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Rabbit PHF8 Polyclonal Antibody | anti-PHF8 antibody
PHF8 Antibody
Gene Names
PHF8; KDM7B; JHDM1F; MRXSSD; ZNF422
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Protein affinity purified
Synonyms
PHF8; Polyclonal Antibody; PHF8 Antibody; Histone lysine demethylase PHF8 antibody; JHDM1F antibody; Jumonji C domain containing histone demethylase 1F antibody; MRXSSD antibody; PHD finger protein 8 antibody; PHF8 antibody; PHF8_HUMAN antibody; ZNF422 antibody; anti-PHF8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Protein affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-PHF8 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Recombinant protein within human PHF8 aa 150-350.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
FCM (Flow Cytometry)
(Flow cytometric analysis of SiHa cells with PHF8 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
FCM (Flow Cytometry)
(Flow cytometric analysis of SiHa cells with PHF8 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry)
(ICC staining PHF8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PHF8 antibody. Counter stained with hematoxylin.)
WB (Western Blot)
(Western blot analysis of PHF8 on different cell lysate using anti-PHF8 antibody at 1/500 dilution. Positive control�� Lane1: PC-3M Lane2: A431 Lane3: Human kidney tissue)
WB (Western Blot)
(Western blot analysis of PHF8 on different cell lysate using anti-PHF8 antibody at 1/500 dilution. Positive control�� Lane1: PC-3M Lane2: A431 Lane3: Human kidney tissue)
Related Product Information for anti-PHF8 antibody
Histone lysine demethylase with selectivity for the di- and monomethyl states that plays a key role cell cycle progression, rDNA transcription and brain development. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2), dimethylated H3 'Lys-27' (H3K27Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Involved in cell cycle progression by being required to control G1-S transition. Required for brain development, probably by regulating expression of neuron-specific genes. Only has activity toward H4K20Me1 when nucleosome is used as a substrate and when not histone octamer is used as substrate. Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: has weak activity toward H3K9Me2 in absence of H3K4me3, while it has high activity toward H3K9me2 when binding H3K4me3.
Product Categories/Family for anti-PHF8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
98,290 Da
NCBI Official Full Name
histone lysine demethylase PHF8 isoform 1
NCBI Official Synonym Full Names
PHD finger protein 8
NCBI Official Symbol
PHF8
NCBI Official Synonym Symbols
KDM7B; JHDM1F; MRXSSD; ZNF422
NCBI Protein Information
histone lysine demethylase PHF8
UniProt Protein Name
Histone lysine demethylase PHF8
UniProt Gene Name
PHF8
UniProt Synonym Gene Names
KIAA1111; ZNF422
Similar Products
Product Notes
The PHF8 phf8 (Catalog #AAA29947) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PHF8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's PHF8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the PHF8 phf8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PHF8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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