WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105) (upper) or Beta-actin (lower).)
Rabbit Phospho-ARHGAP35 (Y1105) Polyclonal Antibody | anti-ARHGAP35 antibody
Phospho-ARHGAP35 (Y1105) antibody
Gene Names
ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Phospho-ARHGAP35 (Y1105), Antibody; Phospho-ARHGAP35 (Y1105) antibody; Rho GTPase-activating protein 35; Glucocorticoid receptor DNA-binding factor 1; Glucocorticoid receptor repression factor 1; GRF-1; Rho GAP p190A; p190-A; ARHGAP35; GRF1; GRLF1; KIAA1722; anti-ARHGAP35 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1090-1135 amino acids from human.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,499
Applicable Applications for anti-ARHGAP35 antibody
ELISA, WB (Western Blot)
Clone Names
RB46920
Antigen Source
HUMAN
Function
Rho GTPase-activating protein (GAP), binds several acidic phospholipids which inhibits the Rho GAP activity to promote the Rac GAP activity (PubMed: ). This binding is inhibited by phosphorylation by PRKCA (PubMed: ). Involved in cell differentiation as well as cell adhesion and migration, plays an important role in retinal tissue morphogenesis, neural tube fusion, midline fusion of the cerebral hemispheres and mammary gland branching morphogenesis (By similarity). Transduces signals from p21-ras to the nucleus, acting via the ras GTPase- activating protein (GAP) (By similarity). Transduces SRC-dependent signal from cell-surface adhesion mo lecules, such as laminin, to promote neurite outgrowth. Regulates axon outgrowth, guidance and fasciculation (By similarity). Modulates Rho GTPase-dependent F- actin polymerization, organization and assembly, is involved in polarized cell migration and in the positive regulation of ciliogenesis and cilia elongation (By similarity). During mammary gland development, is required in both the epithelial and stromal compartments for ductal outgrowth (By similarity). Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'-GAGAAAAGAAACTGGAGAAACTC-3'; this function is however unclear and would need additional experimental evidences (PubMed: ).
Cellular Location
Cytoplasm {ECO:0000250|UniProtKB:Q91YM2}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q91YM2}. Cell membrane {ECO:0000250|UniProtKB:Q91YM2}. Nucleus. Note=In response to integrins and SDC4 and upon phosphorylation by PKC, relocalizes from the cytoplasm to regions of plasma membrane ruffling where it colocalizes with polymerized actin {ECO:0000250|UniProtKB:Q91YM2}
Tissue Location
Detected in neutrophils (at protein level)
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105) (upper) or Beta-actin (lower).)
WB (Western Blot)
(Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using Phospho-HUMAN-ARHGAP35(Y1105) (upper) or Beta-actin (lower).)
Related Product Information for anti-ARHGAP35 antibody
Represses transcription of the glucocorticoid receptor by binding to the cis-acting regulatory sequence 5'- GAGAAAAGAAACTGGAGAAACTC-3'. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTPase-activating protein (GAP). May also act as a tumor suppressor.
Product Categories/Family for anti-ARHGAP35 antibody
References
Nagase T.,et al.DNA Res. 7:347-355(2000).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Tikoo A.,et al.Gene 257:23-31(2000).
LeClerc S.,et al.J. Biol. Chem. 266:17333-17340(1991).
Nakajima D.,et al.DNA Res. 9:99-106(2002).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Tikoo A.,et al.Gene 257:23-31(2000).
LeClerc S.,et al.J. Biol. Chem. 266:17333-17340(1991).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
170514
NCBI Official Full Name
rho GTPase-activating protein 35
NCBI Official Synonym Full Names
Rho GTPase activating protein 35
NCBI Official Symbol
ARHGAP35
NCBI Official Synonym Symbols
GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
NCBI Protein Information
rho GTPase-activating protein 35
UniProt Protein Name
Rho GTPase-activating protein 35
UniProt Gene Name
ARHGAP35
UniProt Synonym Gene Names
GRF1; GRLF1; KIAA1722; GRF-1; p190-A
UniProt Entry Name
RHG35_HUMAN
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Product Notes
The ARHGAP35 arhgap35 (Catalog #AAA286816) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-ARHGAP35 (Y1105) antibody reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-ARHGAP35 (Y1105) can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the ARHGAP35 arhgap35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-ARHGAP35 (Y1105), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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