Rabbit Phospho-CDK7 (T170) Polyclonal Antibody | anti-CDK7 antibody

Phospho-CDK7 (T170) Antibody

Cat. Num. AAA283874

• Gene Names: CDK7; CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• Reactivity: Human (Predicted Reactivity: Mouse)
• Applications: Immunohistochemistry, ELISA, Dot Blot, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Phospho-CDK7 (T170), Antibody; Phospho-CDK7 (T170) Antibody; Cyclin-dependent kinase 7; 39 kDa protein kinase; p39 Mo15; CDK-activating kinase 1; Cell division protein kinase 7; Serine/threonine-protein kinase 1; TFIIH basal transcription factor complex kinase subunit; CDK7; CAK; CAK1; CDKN7; MO15; STK1; anti-CDK7 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This CDK7 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T170 of human CDK7.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 346

• Applicable Applications for anti-CDK7 antibody: IHC (Immunohistochemistry), ELISA, DB (Dot Blot), WB (Western Blot)
• Precautions: Phospho-CDK7 (T170) Antibody is for research use only
• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Colocalizes with PRKCI in the cytoplasm and nucleus (PubMed:15695176). Translocates from the nucleus to cytoplasm and perinuclear region in response to DNA-bound peptides (PubMed:19071173).
• Tissue Location: Ubiquitous.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Formalin-fixed and paraffin-embedded human canccer tissue reacted with the primary antbody, which was peroxidase-conjugated to the secondary antibody, which followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemisty; clinical relevance has not been evaluated. BC= Breast Carcinoma; HC= Hepatocarcinoma.)

DB (Dot Blot)

(Dot blot analysis of Phospho-Cdk7-T170 Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot)

(Western blot analysis of extracts from Hela and MCF-7 cells, untreated or lamda phosphatase-treated, using Phospho-CDK7(please inquire) Antibody (upper) or Beta-actin (lower).)

WB (Western Blot)

(Western blot analysis of lysates from NIH/3T3 cell line, untreated or treated with ? phosphatase, using 459667101(Cat. #AAA283874)(upper) or Tubulin (lower).)

WB (Western Blot)

(The CDK7-T170 Pab is used in Western blot to detect CDK7-T170 in Ramos tissue lysate.)

Related Product Information for anti-CDK7 antibody

Function: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C- terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53; this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Background: The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of Saccharomyces cerevisiae cdc28, and Schizosaccharomyces pombe cdc2, and are known to be important regulators of cell cycle progression. This protein forms a trimeric complex with cyclin H and MAT1, which functions as a Cdk-activating kinase (CAK). It is an essential component of the transcription factor TFIIH, that is involved in transcription initiation and DNA repair. This protein is thought to serve as a direct link between the regulation of transcription and the cell cycle.

Product Categories/Family for anti-CDK7 antibody

Cancer; Cardiovascular; Developmental Biology; Metabolism; Signal Transduction; Phospho Antibodies

References

Zhou, M., et al., Proc. Natl. Acad. Sci. U.S.A. 100(22):12666-12671 (2003).
Kino, T., et al., Biochem. Biophys. Res. Commun. 298(1):17-23 (2002).
Schneider, E., et al., Oncogene 21(33):5031-5037 (2002).
Nekhai, S., et al., Virology 266(2):246-256 (2000).
Zhou, M., et al., Mol. Cell. Biol. 20(14):5077-5086 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 4502743
• NCBI GeneID: 1022
• NCBI Accession #: NP_001790.1
• NCBI GenBank Nucleotide #: NM_001799.3
• UniProt Accession #: P50613; Q9BS60; Q9UE19
• NCBI Official Full Name: cyclin-dependent kinase 7
• NCBI Official Synonym Full Names: cyclin-dependent kinase 7
• NCBI Official Symbol: CDK7
• NCBI Official Synonym Symbols: CAK1; HCAK; MO15; STK1; CDKN7; p39MO15
• NCBI Protein Information: cyclin-dependent kinase 7
• UniProt Protein Name: Cyclin-dependent kinase 7
• UniProt Gene Name: CDK7
• UniProt Synonym Gene Names: CAK; CAK1; CDKN7; MO15; STK1; p39 Mo15
• UniProt Entry Name: CDK7_HUMAN