Rabbit Phospho-INSR (Y1185) Polyclonal Antibody | anti-INSR antibody

Phospho-INSR (Y1185) Antibody

Cat. Num. AAA285280

• Gene Names: INSR; HHF5; CD220
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus, Drosophila)
• Applications: ELISA, Dot Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Phospho-INSR (Y1185), Antibody; Phospho-INSR (Y1185) Antibody; Insulin receptor; IR; CD220; Insulin receptor subunit alpha; Insulin receptor subunit beta; INSR; anti-INSR antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat, Xenopus, Drosophila)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This INSR Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y1185 of human INSR.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 1,370

• Applicable Applications for anti-INSR antibody: ELISA, DB (Dot Blot)
• Function: Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src- homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway, which is responsible for most of the metabolic actions of insulin, and the Ras-MAPK pathway, which regulates expression of some genes and cooperates with the PI3K pathway to control cell growth and differentiation. Binding of the SH2 domains of PI3K to phosphotyrosines on IRS1 leads to the activation of PI3K and the generation of phosphatidylinositol-(3, 4, 5)-triphosphate (PIP3), a lipid second messenger, which activates several PIP3-dependent serine/threonine kinases, such as PDPK1 and subsequently AKT/PKB. The net effect of this pathway is to produce a translocation of the glucose transporter SLC2A4/GLUT4 from cytoplasmic vesicles to the cell membrane to facilitate glucose transport. Moreover, upon insulin stimulation, activated AKT/PKB is responsible for: antiapoptotic effect of insulin by inducing phosphorylation of BAD; regulates the expression of gluconeogenic and lipogenic enzymes by controlling the activity of the winged helix or forkhead (FOX) class of transcription factors. Another pathway regulated by PI3K- AKT/PKB activation is mTORC1 signaling pathway which regulates cell growth and metabolism and integrates signals from insulin. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 thereby activating mTORC1 pathway. The Ras/RAF/MAP2K/MAPK pathway is mainly involved in mediating cell growth, survival and cellular differentiation of insulin. Phosphorylated IRS1 recruits GRB2/SOS complex, which triggers the activation of the Ras/RAF/MAP2K/MAPK pathway. In addition to binding insulin, the insulin receptor can bind insulin-like growth factors (IGFI and IGFII). Isoform Short has a higher affinity for IGFII binding. When present in a hybrid receptor with IGF1R, binds IGF1. PubMed: shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed: shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
• Cellular Location: Cell membrane; Single-pass type I membrane protein
• Tissue Location: Isoform Long and isoform Short are predominantly expressed in tissue targets of insulin metabolic effects: liver, adipose tissue and skeletal muscle but are also expressed in the peripheral nerve, kidney, pulmonary alveoli, pancreatic acini, placenta vascular endothelium, fibroblasts, monocytes,granulocytes, erythrocytes and skin. IsoformShort is preferentially expressed in fetal cellssuch as fetal fibroblasts, muscle, liver andkidney. Found as a hybrid receptor withIGF1R in muscle, heart, kidney, adiposetissue, skeletal muscle, hepatoma,fibroblasts, spleen and placenta (at proteinlevel). Overexpressed in several tumors,including breast, colon, lung, ovary, and thyroid carcinomas.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

DB (Dot Blot)

(Dot blot analysis of anti-Phospho-INSR-pY1185 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Related Product Information for anti-INSR antibody

Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake, thereby mediating the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). This protein can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1. After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked.

Product Categories/Family for anti-INSR antibody

Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction; Phospho Antibodies

References

Nakamaru, K., et al., Biochem. Biophys. Res. Commun. 328(2):449-454 (2005).
Diaz, E., et al., Clin. Biochem. 38(3):243-247 (2005).
McGettrick, A.J., et al., J. Biol. Chem. 280(8):6441-6446 (2005).
Schmitt, T.L., et al., J. Biol. Chem. 280(5):3795-3801 (2005).
Denley, A., et al., Mol. Endocrinol. 18(10):2502-2512 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 119395736
• NCBI GeneID: 3643
• NCBI Accession #: P06213; NP_001073285.1
• NCBI GenBank Nucleotide #: NM_000208.2
• UniProt Accession #: Q9PVZ4; Q17RW0; Q59H98; Q9UCB7; Q9UCB8; Q9UCB9; P06213
• Molecular Weight: 156333
• NCBI Official Full Name: insulin receptor isoform Long preproprotein
• NCBI Official Synonym Full Names: insulin receptor
• NCBI Official Symbol: INSR
• NCBI Official Synonym Symbols: HHF5; CD220
• NCBI Protein Information: insulin receptor
• UniProt Protein Name: Insulin receptor
• UniProt Gene Name: INSR
• UniProt Synonym Gene Names: IR
• UniProt Entry Name: INSR_HUMAN