WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.HeLa cells and C6 cells were treated by MG132(50 uM) at 37 degree C for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit Phospho-SQSTM1/p62-S349 Polyclonal Antibody | anti-SQSTM1/p62-S349 antibody
Phospho-SQSTM1/p62-S349 Rabbit pAb
Gene Names
LOC4330621; MADS57; OsMADS57; OsJ_08264
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purification
Synonyms
Phospho-SQSTM1/p62-S349, Antibody; Phospho-SQSTM1/p62-S349 Rabbit pAb; SQSTM1; A170; DMRV; FTDALS3; NADGP; OSIL; PDB3; ZIP3; p60; p62; p62B; anti-SQSTM1/p62-S349 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Applicable Applications for anti-SQSTM1/p62-S349 antibody
WB (Western Blot)
Positive Samples
HeLa, C6, NIH/3T3
Immunogen
A synthetic phosphorylated peptide around S349 of human SQSTM1/p62 (NP_003891.1).
Cellular Location
Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Late endosome, Lysosome, Nucleus, P-body, autophagosome
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.HeLa cells and C6 cells were treated by MG132(50 uM) at 37 degree C for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of various cell lines, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.HeLa cells and C6 cells were treated by MG132(50 uM) at 37 degree C for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.NIH/3T3 cells were treated by MG132(50 uM) at 37 degree C for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
WB (Western Blot)
(Western blot analysis of extracts of NIH/3T3 cells, using Phospho-SQSTM1/p62-S349 antibody at 1:500 dilution.NIH/3T3 cells were treated by MG132(50 uM) at 37 degree C for 90 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-SQSTM1/p62-S349 antibody
This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone.
Product Categories/Family for anti-SQSTM1/p62-S349 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
27,236 Da
NCBI Official Full Name
MADS-box transcription factor 57 isoform X2
NCBI Official Symbol
LOC4330621
NCBI Official Synonym Symbols
MADS57; OsMADS57; OsJ_08264
NCBI Protein Information
MADS-box transcription factor 57
UniProt Protein Name
MADS-box transcription factor 57
UniProt Gene Name
MADS57
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Product Notes
The SQSTM1/p62-S349 mads57 (Catalog #AAA282220) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-SQSTM1/p62-S349 Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-SQSTM1/p62-S349 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SQSTM1/p62-S349 mads57 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-SQSTM1/p62-S349, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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