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WB (Western Blot)
(Other Applications Image 1 Data: WB Suggested Anti-PPARGC1A antibodyTitration: 0.06 ug/mlPositive Control: SH-SY5Y Neuroblastoma Cells25 ug of the indicated Mouse whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/mL.)
Rabbit PPARGC1A Polyclonal Antibody | anti-PPARGC1A antibody
PPARGC1A antibody - N-terminal region
Gene Names
PPARGC1A; LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
Reactivity
Tested Species Reactivity: HumanPredicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Applications
Western Blot
Purity
Affinity Purified
Synonyms
PPARGC1A, Antibody; PPARGC1A antibody - N-terminal region; anti-PPARGC1A antibody
Host
Rabbit
Reactivity
Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/ml (varies by lot)
Sequence
Synthetic peptide located within the following region: MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDS
Sequence Length
798
Applicable Applications for anti-PPARGC1A antibody
WB (Western Blot)
Homology
Cow: 100%; Dog: 100%; Goat: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 90%
Immunogen
The immunogen is a synthetic peptide directed towards the N terminal region of human PPARGC1A
Protein Size (# AA)
798 amino acids
Protein Interactions
NRIP1; SUMO1; UBE2I; UBC; SIRT1; PPARG; PRKAA1; KAT2A; EGFR; CDK6; CAPNS1; PARK7; PIAS1; SFPQ; ESRRG; RNF2; TP53; HNF4A; CREBZF; NR3C1; ESRRA; RNF34; NR1I2; ESR2; BCL6; SF1; NR5A2; MYEF2; NCOA6; IQGAP1; MYOD1; EP300; LRPPRC; THRB; MYBBP1A; MED1; NR1I3; NC
Enhanced Validation
WB
Y
SPR
YCHAROS
Y
SPR
YCHAROS
Blocking Peptide
For anti-PPARGC1A (MBS3204399) antibody is Catalog #
Preparation and Storage
For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Other Applications Image 1 Data: WB Suggested Anti-PPARGC1A antibodyTitration: 0.06 ug/mlPositive Control: SH-SY5Y Neuroblastoma Cells25 ug of the indicated Mouse whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/mL.)
WB (Western Blot)
(Other Applications Image 1 Data: WB Suggested Anti-PPARGC1A antibodyTitration: 0.06 ug/mlPositive Control: SH-SY5Y Neuroblastoma Cells25 ug of the indicated Mouse whole cell extracts was loaded onto a 6-18% SDS-PAGE gel. 3 ug/mL of the antibody was used in this experiment. Recommended dilution for antibody is 1-3 ug/mL.)
WB (Western Blot)
(WB Suggested Anti-PPARGC1A Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: ACHN cell lysate)
WB (Western Blot)
(WB Suggested Anti-PPARGC1A Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: ACHN cell lysate)
WB (Western Blot)
(PPARGC1A antibody - N-terminal region validated by WB using transfected SH-SY5Y lysate at 1:15000.)
WB (Western Blot)
(PPARGC1A antibody - N-terminal region validated by WB using transfected SH-SY5Y lysate at 1:15000.)
WB (Western Blot)
(PPARGC1A antibody - N-terminal region validated by WB using Transfected Melanoma Cell at 1:1000.)
WB (Western Blot)
(PPARGC1A antibody - N-terminal region validated by WB using Transfected Melanoma Cell at 1:1000.)
WB (Western Blot)
(Lanes:Lane1: 30 ug Human liverLane2: 30 ug Rat liverLane3: 30 ug Mouse (wild-type) liverLane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liverLane5: 30 ug Human muscleLane6: 30 ug Rat muscleLane7: 30 ug Mouse musclePrimary Antibody Dilution:1:1000Secondary Antibody:Secondary Antibody Dilution:1:10000Gene Name:PPARGC1ASubmitted by:Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center)
WB (Western Blot)
(Lanes:Lane1: 30 ug Human liverLane2: 30 ug Rat liverLane3: 30 ug Mouse (wild-type) liverLane4: 30 ug Mouse [AMPK alpha 1/2(-/-)] liverLane5: 30 ug Human muscleLane6: 30 ug Rat muscleLane7: 30 ug Mouse musclePrimary Antibody Dilution:1:1000Secondary Antibody:Secondary Antibody Dilution:1:10000Gene Name:PPARGC1ASubmitted by:Dr. Bruno Guigas, PhD, Dept. of Molecular Cell Biology, Leiden University Medical Center)
Related Product Information for anti-PPARGC1A antibody
Target Description: The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
Product Categories/Family for anti-PPARGC1A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
91kDa
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha isoform 2
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
PPARGC1A
NCBI Official Synonym Symbols
LEM6; PGC1; PGC1A; PGC-1v; PPARGC1; PGC-1alpha; PGC-1(alpha)
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Gene Name
PPARGC1A
UniProt Synonym Gene Names
LEM6; PGC1; PGC1A; PPARGC1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha
UniProt Entry Name
PRGC1_HUMAN
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Product Notes
The PPARGC1A ppargc1a (Catalog #AAA198429) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPARGC1A antibody - N-terminal region reacts with Tested Species Reactivity: Human Predicted Species Reactivity: Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PPARGC1A can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the PPARGC1A ppargc1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: MAWDMCNQDS ESVWSDIECA ALVGEDQPLC PDLPELDLSE LDVNDLDTDS. It is sometimes possible for the material contained within the vial of "PPARGC1A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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