FCM/FACS (Flow Cytometry)
(RAB8A Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human RAB8A Polyclonal Antibody | anti-RAB8A antibody
RAB8A Antibody
Gene Names
RAB8A; MEL; RAB8
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RAB8A, Antibody; RAB8A Antibody; Ras-related protein Rab-8A; Oncogene c-mel; RAB8A; MEL; RAB8; anti-RAB8A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RAB8A antibody is generated from rabbits immunized with human RAB8A recombinant protein.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
207
Applicable Applications for anti-RAB8A antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(RAB8A Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(RAB8A Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB8A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with RAB8A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of RAB8A Antibody in Hela cell line lysates (35ug/lane). RAB8A (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of RAB8A Antibody in Hela cell line lysates (35ug/lane). RAB8A (arrow) was detected using the purified Pab.)
Related Product Information for anti-RAB8A antibody
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Anessential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. RAB8A is a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.
Product Categories/Family for anti-RAB8A antibody
References
Kato,S., et.al., Gene 150 (2), 243-250 (1994)
Bey,F., et.al., Mol. Gen. Genet. 237 (1-2), 193-205 (1993)
Bey,F., et.al., Mol. Gen. Genet. 237 (1-2), 193-205 (1993)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23668
NCBI Official Full Name
ras-related protein Rab-8A
NCBI Official Synonym Full Names
RAB8A, member RAS oncogene family
NCBI Official Symbol
RAB8A
NCBI Official Synonym Symbols
MEL; RAB8
NCBI Protein Information
ras-related protein Rab-8A
UniProt Protein Name
Ras-related protein Rab-8A
UniProt Gene Name
RAB8A
UniProt Synonym Gene Names
MEL; RAB8
UniProt Entry Name
RAB8A_HUMAN
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Product Notes
The RAB8A rab8a (Catalog #AAA288769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RAB8A Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RAB8A can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the RAB8A rab8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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