Goat RAB8A / RAB8 Polyclonal Antibody | anti-RAB8A / RAB8 antibody

Anti-RAB8A / RAB8 Antibody (C-Terminus) IHC-plus

Cat. Num. AAA162221

• Gene Names: RAB8A; MEL; RAB8
• Reactivity: Reactivity: Mouse, Rat, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Applications: Western Blot, Immunofluorescence, Immunohistochemistry
• Purity: Immunoaffinity purified
• Synonyms: RAB8A / RAB8, Antibody; Anti-RAB8A / RAB8 Antibody (C-Terminus) IHC-plus; Goat Polyclonal (IgG) to Mouse RAB8A / RAB8; Mouse RAB8A / RAB8; RAB8A; RAB8; Ras-related protein rab-8; Oncogene c-mel; Ras-associated protein RAB8; Ras-related protein Rab-8A; MEL; anti-RAB8A / RAB8 antibody

• Host: Goat
• Reactivity: Reactivity: Mouse, Rat, Human; Predicted Reactivity: Monkey, Canine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects a band of 24 kDa by Western blot in the following human, rat and mouse whole cell lysates and transfected cells with GFP-Rab8a and GFP-Rab8b cds.
• Purity/Purification: Immunoaffinity purified
• Form/Format: PBS, 20% glycerol, 0.05% sodium azide.
• Concentration: 3 mg/ml (varies by lot)
• Sequence Length: 207

• Applicable Applications for anti-RAB8A / RAB8 antibody: WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry)
• Immunogen: RAB8A/RAB8 antibody was raised against purified recombinant peptide derived from within residues 170 aa to the C-terminus of mouse Rab8a and Rab8b produced in E Coli.
• Immunogen species: RAB8A/RAB8 antibody was raised against Mouse
• Antigen Type: Recombinant protein
• Antigen Modification: C-Terminus
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and AAA Biotech makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

WB (Western Blot)

(Western blot. Anti-Rab8 antibody at 1:500 dilution. Lysates at 50 ug per lane. Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.)

IF (Immunofluorescence)

(Immunofluorescence - anti-Rab8 antibody at 1:100 dilution using primary macrophages. Cells were fixed with 4% of PFA.)

IHC (Immunohistochemistry)

(Anti-RAB8A / RAB8 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Related Product Information for anti-RAB8A / RAB8 antibody

RAB8A Antibody, RAB8 Antibody, Ras-related protein rab-8 Antibody, Oncogene c-mel Antibody, Ras-associated protein RAB8 Antibody, Ras-related protein Rab-8A Antibody, MEL Antibody Description: The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release.

NCBI and Uniprot Product Information

• NCBI GI #: 16933567
• NCBI GeneID: 4218
• NCBI Accession #: NP_005361.2
• NCBI GenBank Nucleotide #: NM_005370.4
• UniProt Accession #: P61006; P24407; Q6FHV5; B4DEK7
• Molecular Weight: 23,564 Da
• NCBI Official Full Name: ras-related protein Rab-8A
• NCBI Official Synonym Full Names: RAB8A, member RAS oncogene family
• NCBI Official Symbol: RAB8A
• NCBI Official Synonym Symbols: MEL; RAB8
• NCBI Protein Information: ras-related protein Rab-8A
• UniProt Protein Name: Ras-related protein Rab-8A
• UniProt Gene Name: RAB8A
• UniProt Synonym Gene Names: MEL; RAB8
• UniProt Entry Name: RAB8A_HUMAN