WB (Western Blot)
(Anti-RAB8A antibody, AAA45709, All Western blottingAll lanes: Anti-RAB8A(AAA45709) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: PC12 Whole Cell Lysate at 40ugLane 6: NIH Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD)
Rabbit RAB8A Polyclonal Antibody | anti-RAB8A antibody
Anti-RAB8A antibody
Gene Names
RAB8A; MEL; RAB8
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
RAB8A, Antibody; Anti-RAB8A antibody; Ras-related protein Rab-8A; RAB8A, member RAS oncogene family; AA409338 antibody; MEL antibody; Mel transforming oncogene(derived from cell line NK14) RAB8 homolog antibody; Mel transforming oncogene(derived from cell line NK14) antibody; Mel transforming oncogene(RAB8 homolog) antibody; Mel transforming oncogene antibody; MGC124948 antibody; Oncogene c mel antibody; Oncogene c-mel antibody; RAB8 antibody; RAB8A antibody; RAB8A member RAS oncogene family antibody; Ras associated protein RAB8 antibody; Ras related protein Rab 8A antibody; Ras-related protein Rab-8A antibody; anti-RAB8A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
207
Applicable Applications for anti-RAB8A antibody
IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RAB8A (170-183aa KAKMDKKLEGNSPQ), identical to the related mouse and rat sequences.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
WB (Western Blot)
(Anti-RAB8A antibody, AAA45709, All Western blottingAll lanes: Anti-RAB8A(AAA45709) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: PC12 Whole Cell Lysate at 40ugLane 6: NIH Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD)
WB (Western Blot)
(Anti-RAB8A antibody, AAA45709, All Western blottingAll lanes: Anti-RAB8A(AAA45709) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 40ugLane 2: Mouse Brain Tissue Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: PC12 Whole Cell Lysate at 40ugLane 6: NIH Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: 293T Whole Cell Lysate at 40ugPredicted bind size: 24KDObserved bind size: 24KD)
IHC (Immunohiostchemistry)
(Anti-RAB8A antibody, AAA45709, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohiostchemistry)
(Anti-RAB8A antibody, AAA45709, IHC(P)IHC(P): Human Mammary Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-RAB8A antibody, AAA45709, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
IHC (Immunohistochemistry)
(Anti-RAB8A antibody, AAA45709, IHC(P)IHC(P): Human Intestinal Cancer Tissue)
Related Product Information for anti-RAB8A antibody
Description: Rabbit IgG polyclonal antibody for Ras-related protein Rab-8A(RAB8A) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. It is mapped to 19p13.12. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. RAB8A regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of RAB8A into the cilium during assembly of this organelle. RAB8A has been shown to interact with Optineurin and MAP4K2.
Background: Ras-related protein Rab-8A is a protein that in humans is encoded by the RAB8A gene. It is mapped to 19p13.12. The protein encoded by this gene is a member of the RAS superfamily which are small GTP/GDP-binding proteins with an average size of 200 amino acids. The RAS-related proteins of the RAB/YPT family may play a role in the transport of proteins from the endoplasmic reticulum to the Golgi and the plasma membrane. RAB8A regulates cilia assembly by targeting and promoting fusion of vesicles near the ciliary membrane. This gene requires CEP290 for centrosome localization and that CEP290 regulates entry of RAB8A into the cilium during assembly of this organelle. RAB8A has been shown to interact with Optineurin and MAP4K2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
23,564 Da
NCBI Official Full Name
Ras-related protein Rab-8A
NCBI Official Synonym Full Names
RAB8A, member RAS oncogene family
NCBI Official Symbol
RAB8A
NCBI Official Synonym Symbols
MEL; RAB8
NCBI Protein Information
ras-related protein Rab-8A; oncogene c-mel; ras-associated protein RAB8; mel transforming oncogene (RAB8 homolog); mel transforming oncogene (derived from cell line NK14); mel transforming oncogene (derived from cell line NK14)- RAB8 homolog
UniProt Protein Name
Ras-related protein Rab-8A
UniProt Gene Name
RAB8A
UniProt Synonym Gene Names
MEL; RAB8
UniProt Entry Name
RAB8A_HUMAN
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Product Notes
The RAB8A rab8a (Catalog #AAA45709) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-RAB8A antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's RAB8A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the RAB8A rab8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RAB8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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