IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded SW480 xenograft using RASGRP4 antibody at a dilution of 1:100)
Rabbit RASGRP4 Polyclonal Antibody | anti-RASGRP4 antibody
RASGRP4 Antibody
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Antigen-Affinity Chromatography
Synonyms
RASGRP4, Antibody; RASGRP4 Antibody; Affinity Purified Rabbit Polyclonal RASGRP4 Antibody; Polyclonal RASGRP4 antibody; Anti-RASGRP4 antibody; guanyl nucleotide releasing protein 4 antibody; RASGRP-4; RAS guanyl releasing protein 4 antibody; RASGRP4 antibody; RASGRP4; RASGRP 4; RASGRP-4 antibody; RAS guanyl-releasing protein 4 antibody; RASGRP 4 antibody; anti-RASGRP4 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen-Affinity Chromatography
Form/Format
Supplied as a concentrated soloution containing 1X PBS, 40% Glycerol (pH 7.0). 0.01% Thimerosal was added as a preservative.
Concentration
1 mg/ml (varies by lot)
Sequence Length
639
Applicable Applications for anti-RASGRP4 antibody
WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence)
Immunogen
RASGRP4 antibody was raised in rabbit using a synthetic peptide corresponding to a region within amino acids 108 and 200 of RASGRP4
Cross-Reactivity
Human
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded SW480 xenograft using RASGRP4 antibody at a dilution of 1:100)
IHC (Immunohistochemisry)
(Immunohistochemical staining of paraffin-embedded SW480 xenograft using RASGRP4 antibody at a dilution of 1:100)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Jurkat) using a 7.5% SDS PAGE gel and RASGRP4 antibody at a dilution of 1:1000)
WB (Western Blot)
(Western blot analysis of 30 ug of whole cell lysate (A: Jurkat) using a 7.5% SDS PAGE gel and RASGRP4 antibody at a dilution of 1:1000)
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4 antibody at 1:500 dilution.)
IF (Immunofluorescence)
(Immunofluorescence analysis of methanol-fixed HeLa, using RASGRP4 antibody at 1:500 dilution.)
Related Product Information for anti-RASGRP4 antibody
The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development.
Product Categories/Family for anti-RASGRP4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75 kDa
NCBI Official Full Name
RAS guanyl-releasing protein 4 isoform e
NCBI Official Synonym Full Names
RAS guanyl releasing protein 4
NCBI Official Symbol
RASGRP4
NCBI Protein Information
RAS guanyl-releasing protein 4
UniProt Protein Name
RAS guanyl-releasing protein 4
UniProt Gene Name
RASGRP4
UniProt Entry Name
GRP4_HUMAN
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Product Notes
The RASGRP4 rasgrp4 (Catalog #AAA225238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's RASGRP4 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ICC (Immunocytochemistry), IF (Immunofluorescence). Researchers should empirically determine the suitability of the RASGRP4 rasgrp4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RASGRP4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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