IF (Immunofluorescence)
(AAA321496 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10%serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321496) and mouse anti-beta tubulinAb for 1 hour at 37°C. An AlexaFluor594 conjugatedgoat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as)
Rabbit Ret Polyclonal Antibody | anti-Ret antibody
Phospho-Ret (Tyr1062) Antibody
Gene Names
RET; PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Synonyms
Ret, Antibody; Phospho-Ret (Tyr1062) Antibody; C ret; Cadherin family member 12; Cadherin related family member 16; CDHF 12; CDHF12; CDHR16; ELKS Fusion gene; HSCR 1; HSCR1; Hydroxyaryl protein kinase; MEN2A; MEN2B; MTC 1; MTC1; Multiple endocrine neoplasia and medullary thyroid carcinoma 1; Oncogene RET; Proto oncogene tyrosine protein kinase receptor ret; Proto-oncogene c-Ret; Proto-oncogene tyrosine-protein kinase receptor ret; PTC; RET; RET ELE1; Ret Proto oncogene; RET transforming sequence; RET_HUMAN; RET51; RET9; tyrosine-protein kinase receptor ret; anti-Ret antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Phospho-Ret (Tyr1062) Antibody detects endogenous levels of Ret only when phosphorylated at Tyrosine 1062.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Ret antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Ret around the phosphorylation site of Tyr1062.
Preparation and Storage
Store at -20°C.
IF (Immunofluorescence)
(AAA321496 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10%serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321496) and mouse anti-beta tubulinAb for 1 hour at 37°C. An AlexaFluor594 conjugatedgoat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as)
IF (Immunofluorescence)
(AAA321496 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10%serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321496) and mouse anti-beta tubulinAb for 1 hour at 37°C. An AlexaFluor594 conjugatedgoat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as)
WB (Western Blot)
(Western blot analysis of Phospho-Ret (Tyr1062) expression in various lysates)
WB (Western Blot)
(Western blot analysis of Phospho-Ret (Tyr1062) expression in various lysates)
WB (Western Blot)
(Western blot analysis of Ret phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot)
(Western blot analysis of Ret phosphorylation expression in K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-Ret antibody
This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
175kDa
NCBI Official Full Name
proto-oncogene tyrosine-protein kinase receptor Ret isoform c
NCBI Official Synonym Full Names
ret proto-oncogene
NCBI Official Symbol
RET
NCBI Official Synonym Symbols
PTC; MTC1; HSCR1; MEN2A; MEN2B; CDHF12; CDHR16; RET-ELE1
NCBI Protein Information
proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Protein Name
Proto-oncogene tyrosine-protein kinase receptor Ret
UniProt Gene Name
RET
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Product Notes
The Ret ret (Catalog #AAA321496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Ret (Tyr1062) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ret can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), WB (Western Blot). Researchers should empirically determine the suitability of the Ret ret for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ret, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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