Rabbit anti-Human, Mouse RFX1 Polyclonal Antibody | anti-RFX1 antibody

Rabbit anti-RFX1 Antibody, Affinity Purified

Cat. Num. AAA212104

• Gene Names: RFX1; EFC; RFX
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Antigen Affinity Purified
• Synonyms: RFX1, Antibody; Rabbit anti-RFX1 Antibody, Affinity Purified; MHC class II regulatory factor RFX; transcription factor RFX1; trans-acting regulatory factor 1; RFX; regulatory factor X, 1 (influences HLA class II expression); Regulatory factor X 1; enhancer factor C; EF-C; EFC; MHC class II regulatory factor RFX1; regulatory factor X1; anti-RFX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
• Concentration: 200ug/ml (varies by lot)

• Applicable Applications for anti-RFX1 antibody: IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
• Immunogen: Between 929 and C-terminus
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

WB (Western Blot)

(Detection of human RFX1 by western blot. Samples: Whole cell lysate (5 ug) from HeLa, MCF-7, HEK293T, K-562, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (AAA212104 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)

WB (Western Blot)

(Detection of mouse RFX1 by western blot. Samples: Whole cell lysate (25 ug) from 3T3-L1, CT26, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RFX1 antibody (AAA212104 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of human RFX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RFX1 antibody (AAA212104 lot 3) used for IP at 6 ug per reaction. RFX1 was also immunoprecipitated by a previous lot of this antibody (AAA212104 lot 2) and a second antibody against a different epitope of RFX1 . For blotting immunoprecipitated RFX1, AAA212104 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohiostchemistry)

(Detection of mouse RFX1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. AAA212104 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry)

(Detection of human RFX1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- RFX1 (Cat. No. AAA212104 Lot3) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB. Counterstain: Hematoxylin (blue).)

Related Product Information for anti-RFX1 antibody

Regulatory factor X, 1 (RFX1) is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X2, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with RFX family members X2, X3, and X5, but not with X4. This protein binds to the X-boxes of MHC class II genes and is essential for their expression [taken from NCBI Entrez Gene (Gene ID: 5989)].

Product Categories/Family for anti-RFX1 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 238859557
• NCBI GeneID: 5989
• NCBI Accession #: NP_002909.4
• NCBI GenBank Nucleotide #: NM_002918.4
• UniProt Accession #: P22670
• Molecular Weight: 104,758 Da
• NCBI Official Full Name: MHC class II regulatory factor RFX1
• NCBI Official Synonym Full Names: regulatory factor X1
• NCBI Official Symbol: RFX1
• NCBI Official Synonym Symbols: EFC; RFX
• NCBI Protein Information: MHC class II regulatory factor RFX1
• UniProt Protein Name: MHC class II regulatory factor RFX1
• UniProt Gene Name: RFX1
• UniProt Synonym Gene Names: EF-C; RFX
• UniProt Entry Name: RFX1_HUMAN