Rabbit anti-Human RFX1 Polyclonal Antibody | anti-RFX1 antibody

RFX1 Antibody

Cat. Num. AAA40838

• Gene Names: RFX1; EFC; RFX
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: RFX1, Antibody; RFX1 Antibody; Regulatory factor X, 1 (influences HLA class II expression); RFX; MHC class II regulatory factor RFX; MHC class II regulatory factor RFX1; enhancer factor C; EFC; trans-acting regulatory factor 1; transcription factor RFX1; anti-RFX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RFX1 antibody is human specific. At least two isoforms of RFX1 are known to exist; this antibody will detect both isoforms.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 979

• Applicable Applications for anti-RFX1 antibody: WB (Western Blot), ELISA
• Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: HeLa Cell Lysate
• Conjugate: Unconjugated
• MW Explanation: Multiple independent RFX1 antibodies also show a band at ~130 kDa
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

ICC (Immunocytochemistry)

(Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 μg/mL.)

IF (Immunofluorescence)

(Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 μg/mL.Green: RFX1 Antibody (8199)Blue: DAPI staining)

ICC (Immunocytochemistry)

(Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 μg/mL.)

WB (Western Blot)

(Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 μg/ml.)

Related Product Information for anti-RFX1 antibody

RFX1 is a member of the regulatory factor X protein family, which are transcription factors that contain a highly-conserved winged helix DNA binding domain and the D region found in the C-terminal part of these proteins which facilitates dimerization (1,2). RFX1 is structurally related to regulatory factors X2, X3, X4, and X5 (3). RFX1 is a regulatory factor essential for MHC class II genes expression and can bind to an inverted repeat that is required for expression of hepatitis B virus genes (4). Recent study identifies RFX1 may play a tumor suppressor role in HCC as autophagy mediator (5).

Product Categories/Family for anti-RFX1 antibody

Immunology; Autophagy

References

Reith W, Ucla C, Barras E, et al. RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins. Mol. Cell Biol. 1994; 14:1230-44. Katan-Khaykovich Y and Shaul Y. RFX1, a single DNA-binding protein with a split dimerization domain, generates alternative complexes. J. Biol. Chem. 1998; 273:24504-12. Hsu YC, Liao WC, Kao CY, et al. Regulation of FGF1 gene promoter through transcription factor RFX1. J. Biol. Chem. 2010; 285:13885-95. Su JC, Tseng PH, Hsu CY, et al. RFX1-dependent activation of SHP-1 induces autophagy by a novel obatoclax derivative in hepatocellular carcinoma cells. Oncotarget 2014; 5:4909-19.

NCBI and Uniprot Product Information

• NCBI GI #: 238859557
• NCBI GeneID: 5989
• NCBI Accession #: NP_002909
• NCBI GenBank Nucleotide #: NM_002918.4
• UniProt Accession #: P22670
• Molecular Weight: Predicted: 108 kDa; Observed: 125 kDa
• NCBI Official Full Name: MHC class II regulatory factor RFX1
• NCBI Official Synonym Full Names: regulatory factor X1
• NCBI Official Symbol: RFX1
• NCBI Official Synonym Symbols: EFC; RFX
• NCBI Protein Information: MHC class II regulatory factor RFX1
• UniProt Protein Name: MHC class II regulatory factor RFX1
• UniProt Gene Name: RFX1
• UniProt Synonym Gene Names: EF-C; RFX
• UniProt Entry Name: RFX1_HUMAN