Rabbit RUNX1/RUNX3 Polyclonal Antibody | anti-RUNX1/RUNX3 antibody

Phospho-RUNX1/RUNX3 (Ser435/Ser397) Antibody

Cat. Num. AAA329259

• Gene Names: RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: RUNX1/RUNX3, Antibody; Phospho-RUNX1/RUNX3 (Ser435/Ser397) Antibody; Acute myeloid leukemia 1; Acute myeloid leukemia 1 protein; alpha subunit core binding factor; AML 1; AML1; AML1 EVI 1; AML1 EVI 1 fusion protein; Aml1 oncogene; AMLCR 1; AMLCR1; CBF alpha 2; CBF-alpha-2; CBFA 2; CBFA2; Core binding factor alpha 2 subunit; Core binding factor runt domain alpha subunit 2; Core-binding factor subunit alpha-2; EVI 1; EVI1; HGNC; Oncogene AML 1; Oncogene AML-1; OTTHUMP00000108696; OTTHUMP00000108697; OTTHUMP00000108699; OTTHUMP00000108700; OTTHUMP00000108702; PEA2 alpha B; PEA2-alpha B; PEBP2 alpha B; PEBP2-alpha B; PEBP2A2; PEBP2aB; Polyomavirus enhancer binding protein 2 alpha B subunit; Polyomavirus enhancer-binding protein 2 alpha B subunit; Run1; Runt related transcription factor 1; Runt-related transcription factor 1; RUNX 1; Runx1; RUNX1_HUMAN; SL3 3 enhancer factor 1 alpha B subunit; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core binding factor alpha B subunit; SL3/AKV core-binding factor alpha B subunit; Acute myeloid leukemia 2 protein; Acute myeloid leukemia gen; anti-RUNX1/RUNX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-RUNX1/RUNX3 (Ser435/Ser397) Antibody detects endogenous levels of RUNX1/RUNX3 only when phosphorylated at Ser435/397.; Tissue Specificity: Expressed in gastric cancer tissues (at protein level).
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-RUNX1/RUNX3 antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry)
• Immunogen: A synthesized peptide derived from human RUNX1/RUNX3 around the phosphorylation site of Ser435/397.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated on tyrosine residues by SRC. Phosphorylated by LCK and FYN.
• Subunit Structure: Heterodimer with CBFB. RUNX3 binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization (By similarity). Interacts with TLE1 and SUV39H1and. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK. Interacts with FOXP3. Interacts with ZFHX3. Interacts with TBX21 (By similarity).
• Similarity: A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes.
• Subcellular Location: Nucleus. Cytoplasm.; Note: The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-RUNX1/RUNX3 antibody

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-beta stimulation. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 49574546
• NCBI GeneID: 861
• NCBI Accession #: NP_001001890.1
• NCBI GenBank Nucleotide #: NM_001001890.2
• UniProt Accession #: Q01196; Q13761
• Molecular Weight: 48,737 Da
• NCBI Official Full Name: runt-related transcription factor 1 isoform AML1b
• NCBI Official Synonym Full Names: runt-related transcription factor 1
• NCBI Official Symbol: RUNX1
• NCBI Official Synonym Symbols: AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1
• NCBI Protein Information: runt-related transcription factor 1; CBF-alpha-2; PEA2-alpha B; PEBP2-alpha B; oncogene AML-1; AML1-EVI-1 fusion protein; acute myeloid leukemia 1 protein; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; core-binding
• UniProt Protein Name: Runt-related transcription factor 1
• UniProt Gene Name: RUNX1
• UniProt Synonym Gene Names: AML1; CBFA2; CBF-alpha-2; PEA2-alpha B
• UniProt Entry Name: RUNX1_HUMAN