IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
Rabbit anti-Human RUNX3 Polyclonal Antibody | anti-RUNX3 antibody
RUNX3 Antibody (Center)
Gene Names
RUNX3; AML2; CBFA3; PEBP2aC
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunohistochemistry, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
RUNX3, Antibody; RUNX3 Antibody (Center); Runt-related transcription factor 3; Acute myeloid leukemia 2 protein; Core-binding factor subunit alpha-3; CBF-alpha-3; Oncogene AML-2; Polyomavirus enhancer-binding protein 2 alpha C subunit; PEA2-alpha C; PEBP2-alpha C; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; RUNX3; AML2; CBFA3; PEBP2A3; anti-RUNX3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This RUNX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-197 amino acids from the Central region of human RUNX3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
168-197
Sequence Length
429
Applicable Applications for anti-RUNX3 antibody
WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA
Function
Forms the heterodimeric complexcore-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the coreconsensus binding sequence 5'- TGTGGT-3',or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain,while CBFB is a non-DNA-binding regulatorysubunit that allosterically enhances thesequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters,including murine leukemia virus,polyomavirus enhancer, T-cell receptorenhancers, LCK, IL3 and GM-CSF promoters(By similarity). May be involved in the controlof cellular proliferation and/or differentiation. In association with ZFHX3, upregulates CDKN1A promoter activity following TGF-betastimulation (PubMed: ). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).
Cellular Location
Nucleus{ECO:0000255|PROSITE-ProRule: PRU00399,ECO:0000269|PubMed:20100835,ECO:0000269|PubMed:20599712}.Cytoplasm. Note=The tyrosine phosphorylated form localizes to the cytoplasm. Translocates from the cytoplasm to the nucleus following TGF-beta stimulation
Tissue Location
Expressed in gastric cancer tissues (at protein level).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
IF (Immunofluorescence)
(Fluorescent confocal image of Hela cell stained with RUNX3 Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RUNX3 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). RUNX3 immunoreactivity is localized to Nucleus and Cytoplasm significantly.)
IHC (Immunohiostchemistry)
(RUNX3 Antibody (Center) (AAA288772)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(RUNX3 Antibody (Center) (AAA288772)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RUNX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center). AAA288772 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HL-60, Jurkat cell line (from left to right), using RUNX3 Antibody (Center). AAA288772 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
Related Product Information for anti-RUNX3 antibody
This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Product Categories/Family for anti-RUNX3 antibody
References
Tsang, Y.H., et al. Oncogene 29(41):5643-5650(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)
Nishio, M., et al. Anticancer Res. 30(7):2673-2682(2010)
Shimada, M., et al. Hum. Genet. 128(4):433-441(2010)
Iwatani, K., et al. Biochem. Biophys. Res. Commun. 400(3):426-431(2010)
Kodach, L.L., et al. Carcinogenesis 31(9):1567-1575(2010)
Nishio, M., et al. Anticancer Res. 30(7):2673-2682(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
runt-related transcription factor 3 isoform 1
NCBI Official Synonym Full Names
runt-related transcription factor 3
NCBI Official Symbol
RUNX3
NCBI Official Synonym Symbols
AML2; CBFA3; PEBP2aC
NCBI Protein Information
runt-related transcription factor 3
UniProt Protein Name
Runt-related transcription factor 3
UniProt Gene Name
RUNX3
UniProt Synonym Gene Names
AML2; CBFA3; PEBP2A3; CBF-alpha-3; PEA2-alpha C; PEBP2-alpha C
UniProt Entry Name
RUNX3_HUMAN
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Product Notes
The RUNX3 runx3 (Catalog #AAA288772) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 168-197. The RUNX3 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's RUNX3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IF (Immunofluorescence), IHC (Immunohistochemistry), ELISA. Researchers should empirically determine the suitability of the RUNX3 runx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "RUNX3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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