FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Scavenging Receptor SR-BI/SCARB1 antibody (AAA126106).Overlay histogram showing MCF-7 cells stained with AAA126106 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 Antibody (AAA126106, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Rabbit anti-Human, Mouse Scavenging Receptor SR-BI/SCARB1 Polyclonal Antibody | anti-SCARB1 antibody
Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband
Gene Names
SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
Reactivity
Human, Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Western Blot
Purity
Immunogen affinity purified.
Synonyms
Scavenging Receptor SR-BI/SCARB1, Antibody; Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband; anti-SCARB1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Natural and recombinant Mouse Ngfr
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration
Adding 0.2 ml of distilled water will yield a concentration of 500 ug/ml. (varies by lot)
Applicable Applications for anti-SCARB1 antibody
ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot)
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
31.2 pg/ml - 2,000 pg/ml
Sensitivity
<10 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at -20 degree C for one year from date of receipt. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for six months. Avoid repeated freezing and thawing.
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Scavenging Receptor SR-BI/SCARB1 antibody (AAA126106).Overlay histogram showing MCF-7 cells stained with AAA126106 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 Antibody (AAA126106, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
FCM/FACS (Flow Cytometry)
(Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-Scavenging Receptor SR-BI/SCARB1 antibody (AAA126106).Overlay histogram showing MCF-7 cells stained with AAA126106 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 Antibody (AAA126106, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
WB (Western Blot)
(Figure 1. Western blot analysis of Scavenging Receptor SR-BI/SCARB1 using anti-Scavenging Receptor SR-BI/SCARB1 antibody (AAA126106).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 antigen affinity purified polyclonal antibody (#AAA126106) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Scavenging Receptor SR-BI/SCARB1 at approximately 85 kDa. The expected band size for Scavenging Receptor SR-BI/SCARB1 is at 85 kDa.)
WB (Western Blot)
(Figure 1. Western blot analysis of Scavenging Receptor SR-BI/SCARB1 using anti-Scavenging Receptor SR-BI/SCARB1 antibody (AAA126106).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human T-47D whole cell lysates,Lane 2: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Scavenging Receptor SR-BI/SCARB1 antigen affinity purified polyclonal antibody (#AAA126106) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Scavenging Receptor SR-BI/SCARB1 at approximately 85 kDa. The expected band size for Scavenging Receptor SR-BI/SCARB1 is at 85 kDa.)
Related Product Information for anti-SCARB1 antibody
Background: Nerve growth factor receptor (NGFR) is also referred to as p75(NTR) because of its ability to bind at low affinity not only to NGF, but also other neurotrophins including brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5. The nerve growth factor receptor gene is at human chromosome region 17q12-17q22, distal to the chromosome 17 breakpoint in acute leukemias. The neurotrophin receptor p75(NTR), a tumor necrosis factor receptor superfamily member expressed in hepatic stellate cells after fibrotic and cirrhotic liver injury in humans, is a regulator of liver repair. In mice, depletion of p75(NTR) exacerbated liver pathology and inhibited hepatocyte proliferation in vivo. In addition, it is showed that neurotrophins activate p75(NTR) to induce apoptosis through the induction of the sphingomyelin (SM) cycle and increased production of ceramide. Overexpression of p75(NTR) is also found to activate the SM pathway.
Principle of the Assay: The Mouse Ngfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ngfr with a 96-well strip plate that is pre-coated with antibody specific for Ngfr. The detection antibody is a biotinylated antibody specific for Ngfr. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Ngfr with immunogen: Expression system for standard: NS0; Immunogen sequence: K22-P246. The kit is analytically validated with ready-to-use reagents. To measure Mouse Ngfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ngfr in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ngfr in the sample.
Principle of the Assay: The Mouse Ngfr Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Mouse Ngfr with a 96-well strip plate that is pre-coated with antibody specific for Ngfr. The detection antibody is a biotinylated antibody specific for Ngfr. The capture antibody is a monoclonal antibody from rat and the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Mouse Ngfr with immunogen: Expression system for standard: NS0; Immunogen sequence: K22-P246. The kit is analytically validated with ready-to-use reagents. To measure Mouse Ngfr, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Mouse Ngfr in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Mouse Ngfr in the sample.
Product Categories/Family for anti-SCARB1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
552
NCBI Official Full Name
Scavenger receptor class B member 1
NCBI Official Synonym Full Names
scavenger receptor class B, member 1
NCBI Official Symbol
SCARB1
NCBI Official Synonym Symbols
CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
NCBI Protein Information
scavenger receptor class B member 1; CD36 and LIMPII analogous 1; scavenger receptor class B type III; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1
UniProt Protein Name
Scavenger receptor class B member 1
UniProt Gene Name
SCARB1
UniProt Synonym Gene Names
CD36L1; CLA1; SRB1; CLA-1
UniProt Entry Name
SCRB1_HUMAN
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Product Notes
The SCARB1 scarb1 (Catalog #AAA126106) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Scavenging Receptor SR-BI/SCARB1 Antibody Picoband reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Scavenging Receptor SR-BI/SCARB1 can be used in a range of immunoassay formats including, but not limited to, ELISA, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the SCARB1 scarb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scavenging Receptor SR-BI/SCARB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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