IP (Immunoprecipitation)
(SRB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate1 uL anti-SRB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SRB1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 81 kDa)
Rabbit anti-Human SR-BI/SCARB1 Polyclonal Antibody | anti-SCARB1 antibody
Anti-SR-BI/SCARB1 Antibody, Rabbit Polyclonal
Gene Names
SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
Reactivity
Human
Applications
Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
SR-BI/SCARB1, Antibody; Anti-SR-BI/SCARB1 Antibody, Rabbit Polyclonal; SR-BI/SCARB1/CD36L1 Antibody, Rabbit PAb, Antigen Affinity Purified; scavenger receptor class B, member 1; Anti-CD36L1 Antibody; Anti-CLA-1 Antibody; Anti-CLA1 Antibody; Anti-HDLQTL6 Antibody; Anti-SR-BI Antibody; Anti-SRB1 Antibody; anti-SCARB1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human SR-BI/SCARB1/CD36L1
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-SCARB1 antibody
IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Recombinant Human SR-BI/SCARB1/CD36L1 Protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human SR-BI/SCARB1/CD36L1 (rh SR-BI/SCARB1/CD36L1). SR-BI/SCARB1/CD36L1 specific IgG was purified by Human SR-BI/SCARB1/CD36L1 affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SRB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate1 uL anti-SRB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SRB1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 81 kDa)
IP (Immunoprecipitation)
(SRB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate1 uL anti-SRB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SRB1 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 60 kDaObserved band size: 81 kDa)
IF (Immunofluorescence)
(Immunofluorescence staining of SRB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SRB1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.)
IF (Immunofluorescence)
(Immunofluorescence staining of SRB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human SRB1 polyclonal antibody (1:5000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm.)
WB (Western Blot)
(Anti-SRB1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:81 kDa(We are unsure as to the identity of these extra bands.))
WB (Western Blot)
(Anti-SRB1 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:60 kDaObserved band size:81 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-SCARB1 antibody
Scavenger receptor class B, member 1 (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. SCARB1 is expressed primarily in liver and non placental steroidogenic tissues, and predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane. SCARB1 is proposed as a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells, and is involved in a wide variety of physilogical processes. As a key component in the reverse cholesterol transport pathway, SCARB1 binds high density lipoproteins (HDLs) and mediates selective cholesterol uptake by a mechanism distinct from the LDL pathway. High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SCARB1 may thus serve as a useful marker that predicts variation in baseline lipid levels and postprandial lipid response. The mouse SCARB1 has been shown to exert actions in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
References
Murao, K. et al., 1997, J. Biol. Chem. 272(28): 17551-17557. Ikemoto, M. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (12): 6538-6543. Husemann, J. et al., 2001, Am. J. Pathol. 158 (3): 825-832. Williams, D.L. et al., 2001, Endocr. Res. 26 (4): 639-651. Bulte, B. S. et al., 2002, J. Biol. Chem. 277 (39): 36092-36099. Duncan, K.G. et al., 2002, Biochem. Biophys. Res. Commun. 292 (4): 1017-1022.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,170 Da
NCBI Official Full Name
scavenger receptor class B member 1 isoform 1
NCBI Official Synonym Full Names
scavenger receptor class B, member 1
NCBI Official Symbol
SCARB1
NCBI Official Synonym Symbols
CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
NCBI Protein Information
scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; scavenger receptor class B type III
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Product Notes
The SCARB1 (Catalog #AAA255313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SR-BI/SCARB1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SR-BI/SCARB1 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the SCARB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SR-BI/SCARB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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