WB (Western Blot)
(Detection of human and mouse SNRNP200 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody AAA212172 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
Rabbit anti-Human, Mouse SNRNP200 Polyclonal Antibody | anti-SNRNP200 antibody
Rabbit anti-SNRNP200 Antibody, Affinity Purified
Gene Names
SNRNP200; BRR2; RP33; HELIC2; ASCC3L1; U5-200KD
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
SNRNP200, Antibody; Rabbit anti-SNRNP200 Antibody, Affinity Purified; BRR2 homolog; small nuclear ribonucleoprotein 200kDa (U5); U5-200KD; U5 snRNP-specific 200 kDa protein; U5 small nuclear ribonucleoprotein 200 kDa helicase; small nuclear ribonucleoprotein, U5 200kDa subunit; HELIC2; activating signal cointegrator 1 complex subunit 3-like 1; BRR2; bad response to refrigeration 2 homolog; ASCC3L1; RP33; small nuclear ribonucleoprotein U5 subunit 200; anti-SNRNP200 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-SNRNP200 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 1 and 50
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse SNRNP200 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody AAA212172 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
WB (Western Blot)
(Detection of human and mouse SNRNP200 by western blot (h and m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody AAA212172 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. SNRNP200 was also immunoprecipitated by rabbit anti-SNRNP200 antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human SNRNP200 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SNRNP200 (Cat. No. AAA212172 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
IHC (Immunohistochemistry)
(Detection of human SNRNP200 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-SNRNP200 (Cat. No. AAA212172 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB. Counterstain: hematoxylin (blue).)
Related Product Information for anti-SNRNP200 antibody
U5 small nuclear ribonucleoprotien 200kDa (SNRNP200) is one of nine U5 snRNP-specific proteins. SNRNP200 belongs to the DEXH-box family of putative RNA helicases. It is a core component of U4/U6-U5 snRNPs and appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices. Mutations in the SNRNP200 gene cause autosomal-dominant retinitis pigmentosa [taken from NCBI Entrez Gene (Gene ID: 23020)].
Product Categories/Family for anti-SNRNP200 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,472 Da
NCBI Official Full Name
U5 small nuclear ribonucleoprotein 200 kDa helicase
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein 200kDa (U5)
NCBI Official Symbol
SNRNP200
NCBI Official Synonym Symbols
BRR2; RP33; HELIC2; ASCC3L1; U5-200KD
NCBI Protein Information
U5 small nuclear ribonucleoprotein 200 kDa helicase
UniProt Protein Name
U5 small nuclear ribonucleoprotein 200 kDa helicase
UniProt Gene Name
SNRNP200
UniProt Synonym Gene Names
ASCC3L1; HELIC2; KIAA0788; U5-200KD
UniProt Entry Name
U520_HUMAN
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Product Notes
The SNRNP200 snrnp200 (Catalog #AAA212172) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-SNRNP200 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's SNRNP200 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the SNRNP200 snrnp200 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRNP200, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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