WB (Western Blot)
(Detection of human STING/TMEM173 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, A-549, K-562, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STING/TMEM173 antibody (AAA213454 lot 2) used for WB at 0.01 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
Rabbit anti-Human STING/TMEM173 Polyclonal Antibody | anti-STING/TMEM173 antibody
Rabbit anti-STING/TMEM173 Antibody, Affinity Purified
Gene Names
TMEM173; ERIS; MITA; MPYS; NET23; STING
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
STING/TMEM173, Antibody; Rabbit anti-STING/TMEM173 Antibody, Affinity Purified; SAVI; Transmembrane protein 173; TMEM173; STING-beta; STING; stimulator of interferon genes protein; N-terminal methionine-proline-tyrosine-serine plasma membrane tetraspanner; NET23; mitochondrial mediator of IRF3 activation; MITA; Mediator of IRF3 activation; hSTING; hMITA; ERIS; endoplasmic reticulum interferon stimulator; endoplasmic reticulum IFN stimulator; MPYS; stimulator of interferon protein; Stimulator of interferon genes protein; transmembrane protein 173; anti-STING/TMEM173 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-STING/TMEM173 antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 329 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human STING/TMEM173 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, A-549, K-562, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STING/TMEM173 antibody (AAA213454 lot 2) used for WB at 0.01 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
WB (Western Blot)
(Detection of human STING/TMEM173 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, A-549, K-562, HEK293T, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-STING/TMEM173 antibody (AAA213454 lot 2) used for WB at 0.01 mg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human STING/TMEM173 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-STING/TMEM173 antibody (AAA213454 lot 2) used for IP at 0.2 ug per reaction. STING/TMEM173 was also immunoprecipitated by a second antibody against a different epitope of STING/TMEM173 (BL24059). For blotting immunoprecipitated STING/TMEM173, AAA213454 was used at 0.01 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human STING/TMEM173 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-STING/TMEM173 antibody (AAA213454 lot 2) used for IP at 0.2 ug per reaction. STING/TMEM173 was also immunoprecipitated by a second antibody against a different epitope of STING/TMEM173 (BL24059). For blotting immunoprecipitated STING/TMEM173, AAA213454 was used at 0.01 mg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
Related Product Information for anti-STING/TMEM173 antibody
STING/TMEM173 is a facilitator of innate immune signaling that acts as a sensor of cytosolic DNA from bacteria and viruses and promotes the production of type I interferon (IFN-alpha and IFN-beta). Innate immune response is triggered in response to non-CpG double-stranded DNA from viruses and bacteria delivered to the cytoplasm. Acts by recognizing and binding cyclic di-GMP (c-di-GMP), a second messenger produced by bacteria, and cyclic GMP-AMP (cGAMP), a messenger produced in response to DNA virus in the cytosol: upon binding of c-di-GMP or cGAMP, autoinhibition is alleviated and TMEM173/STING is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon and exert a potent anti-viral state. May be involved in translocon function, the translocon possibly being able to influence the induction of type I interferons. May be involved in transduction of apoptotic signals via its association with the major histocompatibility complex class II (MHC-II). Mediates death signaling via activation of the extracellular signal-regulated kinase (ERK) pathway. Essential for the induction of IFN-beta in response to human herpes simplex virus 1 (HHV-1) infection. Exhibits 2',3' phosphodiester linkage-specific ligand recognition. Can bind both 2'-3' linked cGAMP and 3'-3' linked cGAMP but is preferentially activated by 2'-3' linked cGAMP. [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q86WV6].
Product Categories/Family for anti-STING/TMEM173 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
379
NCBI Official Full Name
stimulator of interferon genes protein
NCBI Official Synonym Full Names
transmembrane protein 173
NCBI Official Symbol
TMEM173
NCBI Official Synonym Symbols
ERIS; MITA; MPYS; NET23; STING
NCBI Protein Information
stimulator of interferon genes protein; hMITA; hSTING; mediator of IRF3 activation; endoplasmic reticulum IFN stimulator; mitochondrial mediator of IRF3 activation; endoplasmic reticulum interferon stimulator; N-terminal methionine-proline-tyrosine-serine
UniProt Protein Name
Stimulator of interferon genes protein
UniProt Gene Name
TMEM173
UniProt Synonym Gene Names
ERIS; MITA; STING; hSTING; ERIS; hMITA
UniProt Entry Name
STING_HUMAN
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Product Notes
The STING/TMEM173 tmem173 (Catalog #AAA213454) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-STING/TMEM173 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's STING/TMEM173 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the STING/TMEM173 tmem173 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STING/TMEM173, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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