Rabbit TARDBP Polyclonal Antibody | anti-TARDBP antibody

TARDBP Antibody (N-term)

Cat. Num. AAA284138

• Gene Names: TARDBP; ALS10; TDP-43
• Reactivity: Human, Mouse, Rat; Predicted: Chicken
• Applications: ELISA, Immunohistochemistry, Immunofluorescence, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TARDBP, Antibody; TARDBP Antibody (N-term); TAR DNA-binding protein 43; TDP-43; TARDBP; TDP43; anti-TARDBP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Chicken
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TARDBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human TARDBP.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 414

• Applicable Applications for anti-TARDBP antibody: ELISA, IHC (Immunohistochemistry), IF (Immunofluorescence), WB (Western Blot)
• Cellular Location: Nucleus. Cytoplasm. Cytoplasm, Stress granule Note=Continuously travels in and out of the nucleus (PubMed:18957508). Localizes to stress granules in response to oxidative stress (PubMed:19765185).
• Tissue Location: Ubiquitously expressed. In particular, expression is high in pancreas, placenta, lung, genital tract and spleen
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemisry)

(TARDBP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TARDBP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IF (Immunofluorescence)

(Fluorescent confocal image of Hela cell stained with TARDBP Antibody (N-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TARDBP primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).TARDBP immunoreactivity is localized to nucleus significantly and Cytoplasm weakly.)

WB (Western Blot)

(All lanes : Anti-TARDBP Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: A431 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat brain tissue lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-TARDBP antibody

Background: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle.A ctivation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream ofthe transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided byRefSeq].

Function: RNA-binding protein that is involved invarious steps of RNA biogenesis andprocessing (PubMed:23519609).Preferentially binds, via its two RNArecognition motifs RRM1 and RRM2, to GU-repeats on RNA molecules predominantlylocalized within long introns and in the 3'UTRof mRNAs (PubMed:23519609, PubMed:24240615,PubMed:24464995). In turn,regulates the splicing of many non-codingand protein-coding RNAs including proteinsinvolved in neuronal survival, as well asmRNAs that encode proteins relevant forneurodegenerative diseases (PubMed:21358640, PubMed:29438978). Playsa role in maintaining mitochondrialhomeostasis by regulating the processing ofmitochondrial transcripts (PubMed:28794432). Regulatesalso mRNA stability by recruiting CNOT7/CAF1deadenylase on mRNA 3'UTR leading topoly(A) tail deadenylation and thusshortening (PubMed:30520513). Inresponse to oxidative insult, associates withstalled ribosomes localized to stress granules(SGs) and contributes to cell survival(PubMed:23398327, PubMed:19765185).Participates also in the normal skeletalmuscle formation and regeneration, formingcytoplasmic myo-granules and bindingmRNAs that encode sarcomeric proteins(PubMed:30464263).

Product Categories/Family for anti-TARDBP antibody

Crown Antibodies; Neuroscience

References

Kim, S.H., et al. J. Biol. Chem. 285(44):34097-34105(2010)
Geser, F., et al. Arch. Neurol. 67(10):1238-1250(2010)
Mackenzie, I.R., et al. Lancet Neurol 9(10):995-1007(2010)
Shan, X., et al. Proc. Natl. Acad. Sci. U.S.A. 107(37):16325-16330(2010)
McKee, A.C., et al. J. Neuropathol. Exp. Neurol. 69(9):918-929(2010)

NCBI and Uniprot Product Information

• NCBI GI #: 6678271
• NCBI GeneID: 23435
• NCBI Accession #: NP_031401.1
• NCBI GenBank Nucleotide #: NM_007375.3
• UniProt Accession #: Q13148; Q53H27; Q6FI92; Q96DJ0; A4GUK4; A4GUK5; A4GUK6; B2R629; B4DJ45; E2PU12
• NCBI Official Full Name: TAR DNA-binding protein 43
• NCBI Official Synonym Full Names: TAR DNA binding protein
• NCBI Official Symbol: TARDBP
• NCBI Official Synonym Symbols: ALS10; TDP-43
• NCBI Protein Information: TAR DNA-binding protein 43
• UniProt Protein Name: TAR DNA-binding protein 43
• UniProt Gene Name: TARDBP
• UniProt Synonym Gene Names: TDP43; TDP-43
• UniProt Entry Name: TADBP_HUMAN