WB (Western Blot)
(Detection of human and mouse TFE3 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFE3 antibody AAA211945 (lot AAA211945-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse TFE3 Polyclonal Antibody | anti-TFE3 antibody
Rabbit anti-TFE3 Antibody, Affinity Purified
Gene Names
TFE3; TFEA; RCCP2; RCCX1; bHLHe33
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
TFE3, Antibody; Rabbit anti-TFE3 Antibody, Affinity Purified; transcription factor E family, member A; transcription factor for immunoglobulin heavy-chain enhancer 3; transcription factor E3; TFEA; RCCX1; RCCP2; class E basic helix-loop-helix protein 33; bHLHe33; transcription factor for IgH enhancer; Transcription factor E3; transcription factor binding to IGHM enhancer 3; anti-TFE3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-TFE3 antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Bovine
Immunogen
Between 500 and 550
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse TFE3 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFE3 antibody AAA211945 (lot AAA211945-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse TFE3 by western blot. Samples: Whole cell lysate (10 ug) from mIMCD-3, RenCa, NIH 3T3, TCMK-1, and C2C12 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TFE3 antibody AAA211945 (lot AAA211945-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human TFE3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFE3 antibody AAA211945 (lot AAA211945-3) used for IP at 6 ug per reaction. TFE3 was also immunoprecipitated by a previous lot of this antibody (lot AAA211945-2) and rabbit anti-TFE3 antibody For blotting immunoprecipitated TFE3, AAA211945 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TFE3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from A-549 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TFE3 antibody AAA211945 (lot AAA211945-3) used for IP at 6 ug per reaction. TFE3 was also immunoprecipitated by a previous lot of this antibody (lot AAA211945-2) and rabbit anti-TFE3 antibody For blotting immunoprecipitated TFE3, AAA211945 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-TFE3 antibody
Transcription factor E3 (TFE3) is a member of the microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. This family includes four members: MITF, TFE3, TFEB and TFEC. TFE3 activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer [taken from NCBI Entrez Gene (GeneID: 7030)].
Product Categories/Family for anti-TFE3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
61,521 Da
NCBI Official Full Name
transcription factor E3
NCBI Official Synonym Full Names
transcription factor binding to IGHM enhancer 3
NCBI Official Symbol
TFE3
NCBI Official Synonym Symbols
TFEA; RCCP2; RCCX1; bHLHe33
NCBI Protein Information
transcription factor E3; Transcription factor for IgH enhancer; transcription factor E family, member A; class E basic helix-loop-helix protein 33
UniProt Protein Name
Transcription factor E3
UniProt Gene Name
TFE3
UniProt Synonym Gene Names
BHLHE33; bHLHe33
UniProt Entry Name
TFE3_HUMAN
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Product Notes
The TFE3 tfe3 (Catalog #AAA211945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TFE3 Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TFE3 can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TFE3 tfe3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TFE3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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