IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of KIM1 using anti- KIM1 antibody (AAA44922).KIM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- KIM1 Antibody (AAA44922) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Rabbit anti-Rat TIM 1 Polyclonal Antibody | anti-HAVCR1 antibody
Anti-TIM 1 antibody
Gene Names
Havcr1; Kim1; KIM-1
Reactivity
Rat
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunogen affinity purified.
Synonyms
TIM 1, Antibody; Anti-TIM 1 antibody; Hepatitis A virus cellular receptor 1 homolog(HAVcr-1); hepatitis A virus cellular receptor 1; HAVCR 1 antibody; HAVcr-1 antibody; HAVCR1 antibody; Hepatitis A virus cellular receptor 1 antibody; Kidney injury molecule 1 antibody; KIM 1 antibody; KIM-1 antibody; T cell immunoglobin domain and mucin domain protein 1 antibody; T-cell immunoglobulin and mucin domain-containing protein 1 antibody; T-cell membrane protein 1 antibody; TIM antibody; TIM-1 antibody; TIM1 antibody; TIMD 1 antibody; TIMD-1 antibody; TIMD1 antibody; TIMD1_HUMAN antibody; anti-HAVCR1 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
No cross reactivity with other proteins
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized; Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
Sequence Length
307
Applicable Applications for anti-HAVCR1 antibody
IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of rat TIM 1 (289-307aa HPRAEDNIYIIEDRSRGAE).
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of KIM1 using anti- KIM1 antibody (AAA44922).KIM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- KIM1 Antibody (AAA44922) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
IHC (Immunohiostchemistry)
(Figure 2. IHC analysis of KIM1 using anti- KIM1 antibody (AAA44922).KIM1 was detected in paraffin-embedded section of rat testis tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- KIM1 Antibody (AAA44922) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of KIM1 using anti-KIM1 antibody (AAA44922).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIM1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIM1 at approximately 50KD. The expected band size for KIM1 is at 39KD.)
WB (Western Blot)
(Figure 1. Western blot analysis of KIM1 using anti-KIM1 antibody (AAA44922).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat kidney tissue lysates,Lane 2: rat testis tissue lysates,Lane 3: rat heart tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIM1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KIM1 at approximately 50KD. The expected band size for KIM1 is at 39KD.)
Related Product Information for anti-HAVCR1 antibody
KIM1 (KIDNEY INJURY MOLECULE 1), also known as HAVCR1, HAVCR or TIM1, is a protein that in humans is encoded by the KIM1 gene. The KIM1 gene is mapped to 5q33.3. Biochemical, mutational, and cell adhesion analyses confirm that Tim1 is capable of homophilic Tim-Tim interactions. The features identified in murine KIM1 is conserved in human KIM1. The KIM1 protein is indeed a receptor for the virus through the infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV. Using a monoclonal antibody to mouse Tim1, Tim1 is expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. Ectopic expression of KIM1 during mouse T-cell differentiation leads to production of the Th2-type cytokine Il4, but not the Th1-type cytokine Ifng. KIM1-expressing epithelial cells internalized apoptotic bodies, and Kim1 is directly responsible for phagocytosis in cultured primary rat tubule epithelial cells and in porcine and canine epithelial cell lines.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Hepatitis A virus cellular receptor 1 homolog
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 1
NCBI Official Symbol
Havcr1
NCBI Official Synonym Symbols
Kim1; KIM-1
NCBI Protein Information
hepatitis A virus cellular receptor 1 homolog; TIMD-1; HAVcr-1; kidney injury molecule 1; t cell immunoglobulin and mucin domain-containing protein 1
UniProt Protein Name
Hepatitis A virus cellular receptor 1 homolog
UniProt Gene Name
Havcr1
UniProt Synonym Gene Names
Kim1; HAVcr-1; KIM-1; TIMD-1
UniProt Entry Name
HAVR1_RAT
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Product Notes
The HAVCR1 havcr1 (Catalog #AAA44922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIM 1 antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TIM 1 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the HAVCR1 havcr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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