Tim-1 recombinant antibody

Anti-Tim-1 [3B3]

Cat. Num. AAA72045

• Reactivity: Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Protein A Affinity Purified
• Synonyms: Tim-1, Recombinant Antibody; Anti-Tim-1 [3B3]; CD365; HAVcr-1; HAVCR1; Hepatitis A virus cellular receptor 1 homolog; Kidney injury molecule 1; KIM-1; T cell immunoglobulin and mucin domain-containing protein 1; T cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; TIM-1; TIM1; TIMD-1; Tim-1 recombinant antibody

• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG kappa
• Clone Number: 3B3
• Specificity: This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1 has been found on activated CD4+ T cells, mast cells, and a subset of B cells. TIM-1 is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the production of IL-4. TIM-1 can bind several ligands, including Hepatitis A virus, phosphatidylserine, TIM4, IgA, as well as itself.
• Purity/Purification: Protein A Affinity Purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 282

• Applicable Applications for Tim-1 recombinant antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), FCM/FACS (Flow Cytometry)
• Antibody Type: Recombinant Antibody
• Antibody Species: Rabbit
• Original Species Name: Rat
• Immunogen: This antibody was raised by immunising female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse TIM-1–Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting' with mouse TIM-1–Ig in PBS.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.
• Clone Note: This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015), and to study the functions of kidney pericytes in vascular stability (Schrimpf et al, 2012). This antibody has also been used in Western Blot and immunohistochemistry to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and to demonstrate how Bbeta(15-42) attenuates the effect of ischemia-reperfusion injury in renal transplantation (Sörensen et al, 2011). In addition, in vivo applications of this agonistic anti-TIM-1 antibody (clone 3B3) has been shown, for instance, to heighten T cell activation and prevent the development of respiratory tract tolerance in a Th2-driven model of asthma (Umetsu et al, 2005), to increase the frequency of antigen-specific T cells, the production of the proinflammatory cytokines IFN-gamma and IL-17, and thus the severity of experimental autoimmune encephalomyelitis (Sheng et al, 2007), as well as to deprogram Tregs and prevent transplant tolerance in mice (Degauque et al, 2008).
• Buffer Composition: PBS only with 0.02% Proclin 300.
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Application Data

(Surface plasmon resonance (SPR) sensorgram anti-TIM-1 3B3 binding to TIM-1 Fc-fusion protein. Binding of mouse TIM-1 Fc-fusion protein (Pr00227-1.9) to immbolised the rabbit IgG chimeric version of 3B3 (AAA72045). Pr00227-1.9 was injected and allowed to associate with AAA72045 between 1080s and 1250s, before a dissociation phase between 1250s and 1800s. Pr00227-1.9 showed rapid binding to AAA72045 and following ligand removal remained strongly bound. This suggests that AAA72045 has a very high affinity for Pr00227-1.9.)

ELISA

(ELISA of anti-TIM-1 antibody on TIM-1-Fc fusion protein. Binding curves of the rabbit chimeric version of the anti-TIM-1 antibody 3B3 (AAA72045; red line) and isotype control to an ELISA plate coated with mouse TIM-1-Fc fusion protein (Pr00227-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 10000 to 0.17 ng/ml was performed using AAA72045. For signal detection, a 1:4000 dilution of anti-rabbit IgG1 HRP (BioRad) antibody was used.)

Related Product Information for Tim-1 recombinant antibody

Recombinant monoclonal antibody to Tim-1. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma 3B3.

Product Categories/Family for Tim-1 recombinant antibody

Immunology; CD Markers; Cell Signalling; T Cells

References

Umetsu Sarah et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nat Immunol. 2005 May;6(5):447-54. PMID:15793575
Original Published Journal: Nat Immunol. 2005 May;6(5):447-54.
Publication Note: Describe the original generation of this antibody and its use in FACS analysis and in a TCR-transgenic DO11.10 mouse model to demonstrate that TIM-1 induces T cell activation and inhibits the development of peripheral tolerance.

NCBI and Uniprot Product Information

• NCBI GI #: 262231823
• NCBI Accession #: NP_001160103.1
• NCBI GenBank Nucleotide #: NP_001160103.1
• UniProt Accession #: Q5QNS5
• NCBI Official Full Name: hepatitis A virus cellular receptor 1 homolog isoform b
• UniProt Protein Name: Hepatitis A virus cellular receptor 1 homolog
• UniProt Gene Name: Havcr1
• UniProt Synonym Gene Names: Kim1; Tim1; Timd1; HAVcr-1; KIM-1; TIMD-1; TIM-1
• UniProt Entry Name: HAVR1_MOUSE