WB (Western Blot)
(Figure 2. WB Validation in Rat Liver Loading: 15 ?g of rat liver lysate Antibodies: TIM-1 9927, 2 ?g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
Rabbit anti-Mouse, rat TIM-1 Polyclonal Antibody | anti-TIM-1 antibody
TIM-1 Antibody
Reactivity
Mouse, rat
Applications
Western Blot, ELISA
Purity
TIM-1 Antibody is affinity chromatography purified via peptide column.
Synonyms
TIM-1, Antibody; TIM-1 Antibody; anti-TIM-1 antibody
Host
Rabbit
Reactivity
Mouse, rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
TIM-1 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Applicable Applications for anti-TIM-1 antibody
WB (Western Blot), ELISA
Immunogen
Anti-TIM-1 antibody (9927) was raised against a peptide corresponding to 16 amino acids near the amino terminus of mouse TIM-1.
The immunogen is located within amino acids 50 - 100 of TIM-1.
The immunogen is located within amino acids 50 - 100 of TIM-1.
Isoform
Mouse TIM-1 has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1 has only one isoform identified so far (307aa, 34kD).
Buffer
TIM-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
TIM-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
WB (Western Blot)
(Figure 2. WB Validation in Rat Liver Loading: 15 ?g of rat liver lysate Antibodies: TIM-1 9927, 2 ?g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
WB (Western Blot)
(Figure 2. WB Validation in Rat Liver Loading: 15 ?g of rat liver lysate Antibodies: TIM-1 9927, 2 ?g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.)
Application Data
(Figure 1. Relative Expression Validation of TIM-1 in Mouse Tissues Loading: 15 ?g of mouse tissue lysate of each lane Antibodies: TIM-1 9927, 2 ?g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas.)
Application Data
(Figure 1. Relative Expression Validation of TIM-1 in Mouse Tissues Loading: 15 ?g of mouse tissue lysate of each lane Antibodies: TIM-1 9927, 2 ?g/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.TIM-1 antibody (9927) detected high expression levels of TIM-1 in the mouse kidney and testis, while showing very low or no expression in the lung, brain and thymus. This pattern aligns with the RNA expression data for these tissues in human, as reported by Human Protein Atlas.)
Related Product Information for anti-TIM-1 antibody
TIM-1 Antibody: The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
Product Categories/Family for anti-TIM-1 antibody
References
Feigelstock et al. J. Virol. 1998; 72:6621-8
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Product Notes
The TIM-1 (Catalog #AAA41015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TIM-1 Antibody reacts with Mouse, rat and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-1 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the TIM-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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