Rabbit TOLLIP Polyclonal Antibody | anti-TOLLIP antibody

TOLLIP Antibody

Cat. Num. AAA168199

• Gene Names: TOLLIP; IL-1RAcPIP
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antigen affinity purification
• Synonyms: TOLLIP, Antibody; TOLLIP Antibody; FLJ33531; IL-1RAcPIP; anti-TOLLIP antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 274

• Applicable Applications for anti-TOLLIP antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: Recombinant protein of human TOLLIP
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-292758
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cell using TOLLIP antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human stomach using TOLLIP antibody at dilution of 1:200 (x400 lens))

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using TOLLIP antibody.)

Related Product Information for anti-TOLLIP antibody

Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses (1-3). TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-kappaB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. Activation of IKK leads to the degradation of IkappaB, which normally maintains NF-kappaB in an inactive state by sequestering it in the cytoplasm. Tollip (Toll interacting protein) is an adaptor protein discovered to be associated with the IRAK complex and recruited to IL1-R following IL-1 stimulation (4). Overexpression of Tollip results in impaired NF-kappaB signaling (4). Tollip also associates directly with TLR2 and TLR4 and inhibits TLR-mediated signaling through inhibition of IRAK (5). Studies of Tollip deficient mice suggest that it plays a role in the regulation of inflammatory cytokines in response to IL-1 and LPS (6).

NCBI and Uniprot Product Information

• NCBI GI #: 21361619
• NCBI GeneID: 54472
• NCBI Accession #: NP_061882.2
• NCBI GenBank Nucleotide #: NM_019009.3
• Molecular Weight: 22,812 Da
• NCBI Official Full Name: toll-interacting protein
• NCBI Official Synonym Full Names: toll interacting protein
• NCBI Official Symbol: TOLLIP
• NCBI Official Synonym Symbols: IL-1RAcPIP
• NCBI Protein Information: toll-interacting protein; adapter protein
• UniProt Protein Name: Toll-interacting protein
• UniProt Gene Name: TOLLIP
• UniProt Entry Name: TOLIP_HUMAN