ICC (Immunocytochemistry)
(ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).)
Rabbit TOP2A Polyclonal Antibody | anti-TOP2A antibody
TOP2A Antibody
Gene Names
Top2a; Top-2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Immunogen affinity purified
Synonyms
TOP2A, Antibody; TOP2A Antibody; alpha isozyme antibody; ATP hydrolyzing DNA topoisomerase II alfa antibody; DNA gyrase antibody; DNA topoisomerase (ATP hydrolyzing) antibody; DNA topoisomerase 2 alpha antibody; DNA topoisomerase 2-alpha antibody; DNA topoisomerase II 170 kD antibody; DNA topoisomerase II alpha isozyme antibody; DNA topoisomerase II antibody; DNA Topoisomerase2 antibody; TOP 2A antibody; TOP2 antibody; TOP2A antibody; TOP2A_HUMAN antibody; Topoisomerase DNA II alpha 170kDa antibody; TP2A antibody; anti-TOP2A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-TOP2A antibody
ICC (Immunocytochemistry), WB (Western Blot)
Immunogen
Recombinant protein
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.
ICC (Immunocytochemistry)
(ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).)
ICC (Immunocytochemistry)
(ICC staining TOP2A in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).)
WB (Western Blot)
(Western blot analysis on Jurkat cell lysates using anti-TOP2A rabbit polyclonal antibodies.)
WB (Western Blot)
(Western blot analysis on Jurkat cell lysates using anti-TOP2A rabbit polyclonal antibodies.)
Related Product Information for anti-TOP2A antibody
DNA topoisomerases I and II are nuclear enzymes; type II consists of two highly homologous isoforms: topoisomerase IIalpha and IIbeta . This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Topoisomerases are very susceptible to various stresses. Acidic pH or oxidative stress can convert topoisomerases to DNA-breaking nucleases, causing genomic instability and cell death. DNA-damaging topoisomerase targeting drugs (e.g., etoposide) also convert topoisomerases to nucleases, with the enzyme usually trapped as an intermediate that is covalently bound to the 5+ end of the cleaved DNA strand (s).
Product Categories/Family for anti-TOP2A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
172,790 Da
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha
NCBI Official Symbol
Top2a
NCBI Official Synonym Symbols
Top-2
NCBI Protein Information
DNA topoisomerase 2-alpha
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
Top2a
UniProt Synonym Gene Names
Top-2; Top2
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Product Notes
The TOP2A top2a (Catalog #AAA311928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP2A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TOP2A can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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