IP (Immunoprecipitation)
(SECTM1 was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-SECTM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SECTM1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 174 kDa; Observed band size :180 kDa)
Rabbit anti-Human TOP2A Polyclonal Antibody | anti-TOP2A antibody
TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Synonyms
TOP2A, Antibody; TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-TOP2 Antibody; Anti-TP2A Antibody; anti-TOP2A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Human TOP2A
Applicable Applications for anti-TOP2A antibody
IP (Immunoprecipitation), WB (Western Blot)
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human TOP2A, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(SECTM1 was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-SECTM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SECTM1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 174 kDa; Observed band size :180 kDa)
IP (Immunoprecipitation)
(SECTM1 was immunoprecipitated using:; Lane A:0.5 mg Jurkat Whole Cell Lysate; 4 uL anti-SECTM1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.; Primary antibody:; Anti-SECTM1 rabbit polyclonal antibody,at 1:100 dilution; Secondary antibody:; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size: 174 kDa; Observed band size :180 kDa)
WB (Western Blot)
(Anti-TOP2A rabbit polyclonal antibody at 1:500 dilution; Lane A: HeLa Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:174 kDa)
WB (Western Blot)
(Anti-TOP2A rabbit polyclonal antibody at 1:500 dilution; Lane A: HeLa Whole Cell Lysate; Lane B: Jurkat Whole Cell Lysate; Lysates/proteins at 30 ug per lane.; Secondary; Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.; Developed using the ECL technique.; Performed under reducing conditions.; Predicted band size:174 kDa)
Related Product Information for anti-TOP2A antibody
Type II topoisomerases orchestrate proper DNA topology, and they are the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations. TOP2A cleavage as a broad DNA damage mechanism in oncogenic translocations as well as a functional role of TOP2A cleavage in regulating transcription elongation and gene activation. It has been reported that Topoisomerase II alpha (TOP2A) could induce tumor development and progression in many cancer types. The up-regulation of TOP2A was significantly correlated with tumor metastasis and shorter survival in patients with pancreatic cancer. TOP2A plays an important role in the tumorigenesis of many malignancies, such as breast cancer, ovarian cancer, and prostate cancer, and TOP2A may serve as an oncogene in colon cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
174,385 Da
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
UniProt Entry Name
TOP2A_HUMAN
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Product Notes
The TOP2A top2a (Catalog #AAA258606) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TOP2A Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TOP2A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TOP2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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