WB (Western Blot)
(Detection of human TOPO II Alpha by western blot. Samples: Whole cell lysate (10 ug) from K-562, HeLa, A-549, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPO II Alpha antibody (AAA210740 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human Topo II Alpha Polyclonal Antibody | anti-TopoIIAlpha antibody
Rabbit anti-Topo II Alpha Antibody, Affinity Purified
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Topo II Alpha, Antibody; Rabbit anti-Topo II Alpha Antibody, Affinity Purified; DNA topoisomerase II, alpha isozyme; TP2A; DNA gyrase; TOP2; DNA topoisomerase II, 170 kD; DNA topoisomerase 2-alpha; DNA topoisomerase (ATP-hydrolyzing); topoisomerase (DNA) II alpha 170kDa; DNA topoisomerase II alpha; anti-TopoIIAlpha antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TopoIIAlpha antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1481 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human TOPO II Alpha by western blot. Samples: Whole cell lysate (10 ug) from K-562, HeLa, A-549, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPO II Alpha antibody (AAA210740 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human TOPO II Alpha by western blot. Samples: Whole cell lysate (10 ug) from K-562, HeLa, A-549, Ramos, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-TOPO II Alpha antibody (AAA210740 lot 3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human TOPO II Alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOPO II Alpha antibody (AAA210740 lot 3) used for IP at 6 ug per reaction. TOPO II Alpha was also immunoprecipitated by a previous lot of this antibody (AAA210740 lot 2). For blotting immunoprecipitated TOPO II Alpha, AAA210740 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TOPO II Alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from K-562 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TOPO II Alpha antibody (AAA210740 lot 3) used for IP at 6 ug per reaction. TOPO II Alpha was also immunoprecipitated by a previous lot of this antibody (AAA210740 lot 2). For blotting immunoprecipitated TOPO II Alpha, AAA210740 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IHC (Immunohistochemistry)
(Detection of human Topo II Alpha by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II alpha (AAA210740) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Topo II Alpha by immunohistochemistry. Sample: FFPE section of human laryngeal squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Topo II alpha (AAA210740) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-TopoIIAlpha antibody
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
Product Categories/Family for anti-TopoIIAlpha antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
174,385 Da
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha 170kDa
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha; DNA gyrase; DNA topoisomerase II, 170 kD; DNA topoisomerase (ATP-hydrolyzing); DNA topoisomerase II, alpha isozyme
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
UniProt Entry Name
TOP2A_HUMAN
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Product Notes
The TopoIIAlpha top2a (Catalog #AAA210740) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Topo II Alpha Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Topo II Alpha can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TopoIIAlpha top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topo II Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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