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WB (Western Blot)
(Detection of human and mouse Topo II Beta by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody AAA211242 (lot AAA211242-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse Topo II Beta Polyclonal Antibody | anti-TopoIIBeta antibody
Rabbit anti-Topo II Beta Antibody, Affinity Purified
Gene Names
TOP2B; TOPIIB; top2beta
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
Topo II Beta, Antibody; Rabbit anti-Topo II Beta Antibody, Affinity Purified; topo II beta; antigen MLAA-44; topoisomerase IIb; U937 associated antigen; topoisomerase II beta; topoisomerase (DNA) II beta 180kDa; top2beta; DNA topoisomerase II, beta isozyme; DNA topoisomerase 2-beta; DNA topoisomerase II, 180 kD; TOPIIB; DNA topoisomerase II beta; anti-TopoIIBeta antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-TopoIIBeta antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1576 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse Topo II Beta by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody AAA211242 (lot AAA211242-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse Topo II Beta by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Topo II Beta antibody AAA211242 (lot AAA211242-2) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human Topo II Beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody AAA211242 (lot AAA211242-2) used for IP at 6 ug per reaction. Topo II Beta was also immunoprecipitated by a previous lot of this antibody (lot AAA211242-1) and rabbit anti-Topo II Beta antibody For blotting immunoprecipitated Topo II Beta, AAA211242 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human Topo II Beta by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Topo II Beta antibody AAA211242 (lot AAA211242-2) used for IP at 6 ug per reaction. Topo II Beta was also immunoprecipitated by a previous lot of this antibody (lot AAA211242-1) and rabbit anti-Topo II Beta antibody For blotting immunoprecipitated Topo II Beta, AAA211242 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IHC (Immunohiostchemistry)
(Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta (Cat. No. AAA211242 lot 2) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human ovarian cancer. Antibody: Affinity purified rabbit anti-Topo II Beta (Cat. No. AAA211242 lot 2) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta (Cat. No. AAA211242 lot 2) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human Topo II Beta by immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-Topo II Beta (Cat. No. AAA211242 lot 2) used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-TopoIIBeta antibody
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II beta is one of two described isoforms that are likely products of a gene duplication event. TopoII beta is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.
Product Categories/Family for anti-TopoIIBeta antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
183,267 Da
NCBI Official Full Name
DNA topoisomerase 2-beta
NCBI Official Synonym Full Names
topoisomerase (DNA) II beta 180kDa
NCBI Official Symbol
TOP2B
NCBI Official Synonym Symbols
TOPIIB; top2beta
NCBI Protein Information
DNA topoisomerase 2-beta; topo II beta; antigen MLAA-44; topoisomerase IIb; topoisomerase II beta; U937 associated antigen; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme
UniProt Protein Name
DNA topoisomerase 2-beta
UniProt Gene Name
TOP2B
UniProt Entry Name
TOP2B_HUMAN
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Product Notes
The TopoIIBeta top2b (Catalog #AAA211242) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Topo II Beta Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Topo II Beta can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the TopoIIBeta top2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topo II Beta, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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