WB (Western Blot)
(Expression analysis of Topo II alpha. Anti-Topo II alpha antibody (Clone: ABM48B4) was tested at 1 ug/ml on (1) HT-29, (2) 293, (3) A549 and (4) Raji Lysates.)
Mouse anti-Human Topo II alpha Monoclonal Antibody | anti-TOP2A antibody
Topo II alpha
Gene Names
TOP2A; TOP2; TP2A
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Protein G purification
Synonyms
Topo II alpha, Antibody; Topo II alpha; anti-TOP2A antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b, Kappa
Clone Number
ABM48B4
Purity/Purification
Protein G purification
Form/Format
Purified
Concentration
0.5 mg/ml (varies by lot)
Sequence Length
1531
Applicable Applications for anti-TOP2A antibody
FCM/FACS (Flow Cytometry), WB (Western Blot)
Immunogen
A partial length recombinant human Topo II alpha protein (amino acids 1326-1512) was used as the immunogen for this antibody.
Content
25 ug in 50 ul/100 ug in 200 ?l PBS containing 0.05% BSA and 0.05% sodium azide. Sodium
azide is highly toxic.
Target Modification
No
Produced from Ascites
No
Preparation and Storage
Store the antibody at 4 degree C, stable for 6 months. For long-term storage, store at -20 degree C. Avoid repeated freeze and thaw cycles.
WB (Western Blot)
(Expression analysis of Topo II alpha. Anti-Topo II alpha antibody (Clone: ABM48B4) was tested at 1 ug/ml on (1) HT-29, (2) 293, (3) A549 and (4) Raji Lysates.)
WB (Western Blot)
(Expression analysis of Topo II alpha. Anti-Topo II alpha antibody (Clone: ABM48B4) was tested at 1 ug/ml on (1) HT-29, (2) 293, (3) A549 and (4) Raji Lysates.)
FCM/FACS (Flow Cytometry)
(Intracellular Flow analysis of Topo II alpha antibody in Jurkat cells using 0.5 ug/10^6 cells of anti-Topo II alpha antibody (ABM48B4). Green represents isotype control;red represents anti-Topo II alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
FCM/FACS (Flow Cytometry)
(Intracellular Flow analysis of Topo II alpha antibody in Jurkat cells using 0.5 ug/10^6 cells of anti-Topo II alpha antibody (ABM48B4). Green represents isotype control;red represents anti-Topo II alpha antibody. Goat anti-mouse PE conjugate was used as secondary antibody.)
Related Product Information for anti-TOP2A antibody
Topoisomerases are essential nuclear enzymes that are involved in DNA supercoiling regulation and play key roles in transcription, replication, and chromosome segregation. Two major classes of topoisomerases (types I and II) are distinguished by the number of DNA strands that they cleave and the mechanism by which they alter the topological properties of DNA. Topo II Alpha is cell cycle-regulated and is essential for the survival of proliferating cells. It accumulates on chromatin during M-phase, a dynamic localization that is dependent on its C-terminal domain. Although Topo II alpha is the major form of Topo II responsible for decatenation, mitotic chromosome formation and chromosome segregation in proliferating cells, the contribution of the two isoforms has not yet been fully established. Topo II Alpha plays an important role in DNA synthesis and transcription and has been implicated in a variety of human cancers. It also plays a role in various cancer subtypes and acts as targets for cancer therapy, as well as biomarkers for prediction of response.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
DNA topoisomerase 2-alpha
NCBI Official Synonym Full Names
topoisomerase (DNA) II alpha
NCBI Official Symbol
TOP2A
NCBI Official Synonym Symbols
TOP2; TP2A
NCBI Protein Information
DNA topoisomerase 2-alpha
UniProt Protein Name
DNA topoisomerase 2-alpha
UniProt Gene Name
TOP2A
UniProt Synonym Gene Names
TOP2
UniProt Entry Name
TOP2A_HUMAN
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Product Notes
The TOP2A top2a (Catalog #AAA78248) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Topo II alpha reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Topo II alpha can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), WB (Western Blot). Researchers should empirically determine the suitability of the TOP2A top2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Topo II alpha, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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