IP (Immunoprecipitation)
(Detection of human TRAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRAF2 antibody AAA212176 used for IP at 6 ug/mg lysate. TRAF2 was also immunoprecipitated by rabbit anti-TRAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated TRAf2, was used at 0.4 ug/ml,. Detection: Chemiluminescence with an exposure time 10 seconds.)
Rabbit anti-Human TRAF2 Polyclonal Antibody | anti-TRAF2 antibody
Rabbit anti-TRAF2 Antibody, Affinity Purified
Gene Names
TRAF2; TRAP; TRAP3; MGC:45012
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation
Purity
Antigen Affinity Purified
Synonyms
TRAF2, Antibody; Rabbit anti-TRAF2 Antibody, Affinity Purified; TRAP; Tumor necrosis factor type 2 receptor-associated protein 3; tumor necrosis factor type 2 receptor associated protein 3; TRAP3; RNF117; RING-type E3 ubiquitin transferase TRAF2; MGC:45012; E3 ubiquitin-protein ligase TRAF2; TNF receptor-associated factor 2; TNF receptor associated factor 2; anti-TRAF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-TRAF2 antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation)
Predicted Reactivity
Mouse
Immunogen
Between 300 and 350
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
IP (Immunoprecipitation)
(Detection of human TRAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRAF2 antibody AAA212176 used for IP at 6 ug/mg lysate. TRAF2 was also immunoprecipitated by rabbit anti-TRAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated TRAf2, was used at 0.4 ug/ml,. Detection: Chemiluminescence with an exposure time 10 seconds.)
IP (Immunoprecipitation)
(Detection of human TRAF2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-TRAF2 antibody AAA212176 used for IP at 6 ug/mg lysate. TRAF2 was also immunoprecipitated by rabbit anti-TRAF2 antibody which recognizes an upstream epitope. For blotting immunoprecipitated TRAf2, was used at 0.4 ug/ml,. Detection: Chemiluminescence with an exposure time 10 seconds.)
IHC (Immunohiostchemistry)
(Detection of human TRAF2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAF2 (Cat. No. AAA212176 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohiostchemistry)
(Detection of human TRAF2 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-TRAF2 (Cat. No. AAA212176 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TRAF2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TRAF2 (Cat. No. AAA212176 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human TRAF2 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-TRAF2 (Cat. No. AAA212176 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)
Related Product Information for anti-TRAF2 antibody
TNF receptor-associated factor 2 (TRAF2) regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. TRAF2 is required for normal antibody isotype switching from IgM to IgG. TRAF2 has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. TRAF2 is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q12933].
Product Categories/Family for anti-TRAF2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,859 Da
NCBI Official Full Name
TNF receptor-associated factor 2
NCBI Official Synonym Full Names
TNF receptor-associated factor 2
NCBI Official Symbol
TRAF2
NCBI Official Synonym Symbols
TRAP; TRAP3; MGC:45012
NCBI Protein Information
TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; tumor necrosis factor type 2 receptor associated protein 3; tumor necrosis factor type 2 receptor-associated protein 3
UniProt Protein Name
TNF receptor-associated factor 2
UniProt Gene Name
TRAF2
UniProt Synonym Gene Names
TRAP3
UniProt Entry Name
TRAF2_HUMAN
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Product Notes
The TRAF2 traf2 (Catalog #AAA212176) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-TRAF2 Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRAF2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation). Researchers should empirically determine the suitability of the TRAF2 traf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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