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KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: VF knockout 293T cell lysate; Predicted MW: 55kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Rabbit Visfatin Polyclonal Antibody | anti-VF antibody
Polyclonal Antibody to Visfatin (VF)
Gene Names
NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
Reactivity
Human, Mouse, Rat, Pig
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Visfatin, Antibody; Polyclonal Antibody to Visfatin (VF); anti-VF antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Pig
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against VF. It has beenselected for its ability to recognize VF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.6mg/ml (varies by lot)
Sequence Length
491
Applicable Applications for anti-VF antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
Fragment
Visfatin (Met1~His491)
Organism Species
Homo sapiens (Human)
Cross Reactivity
Human, Pig
Conjugate
No Conjugate
Immunogen
Recombinant Visfatin (Met1~His491) expressed in E Coli.
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: VF knockout 293T cell lysate; Predicted MW: 55kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
KO (Knockout Validation)
(Knockout Validation: Lane 1: Wild-type 293T cell lysate; Lane 2: VF knockout 293T cell lysate; Predicted MW: 55kd Observed MW: 55kd Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
IHC (Immunohistchemistry)
(DAB staining on IHC-P. Samples: Human Tissue))
IHC (Immunohistchemistry)
(DAB staining on IHC-P. Samples: Human Tissue))
WB (Western Blot)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Human 293T cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Recombinant protein.)
WB (Western Blot)
(Western Blot: Sample: Porcine Stomach lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Stomach lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Heart lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
WB (Western Blot)
(Western Blot: Sample: Porcine Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Human VF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)
Product Categories/Family for anti-VF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,521 Da
NCBI Official Full Name
nicotinamide phosphoribosyltransferase
NCBI Official Synonym Full Names
nicotinamide phosphoribosyltransferase
NCBI Official Symbol
NAMPT
NCBI Official Synonym Symbols
VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
NCBI Protein Information
nicotinamide phosphoribosyltransferase
UniProt Protein Name
Nicotinamide phosphoribosyltransferase
UniProt Gene Name
NAMPT
UniProt Synonym Gene Names
PBEF; PBEF1; NAmPRTase; Nampt; Pre-B cell-enhancing factor
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Product Notes
The VF nampt (Catalog #AAA20862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Visfatin (VF) reacts with Human, Mouse, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's Visfatin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.2-2ug/mL;1:250-2500 Immunohistochemistry: 5-20ug/mL;1:25-100 Immunocytochemistry: 5-20ug/mL;1:25-100 Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the VF nampt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Visfatin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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