Rabbit XBP1 Polyclonal Antibody | anti-XBP1 antibody

XBP1 Antibody (Center)

Cat. Num. AAA284859

• Gene Names: XBP1; XBP2; TREB5; XBP-1; TREB-5
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: XBP1, Antibody; XBP1 Antibody (Center); X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5; XBP1; TREB5; XBP2; anti-XBP1 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine, Mouse, Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This XBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-91 amino acids from the Central region of human XBP1.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 62-91
• Sequence Length: 376

• Applicable Applications for anti-XBP1 antibody: FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(Western blot analysis of XBP1 (arrow) using rabbit polyclonal XBP1 Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the XBP1 gene.)

FCM/FACS (Flow Cytometry)

(XBP1 Antibody (Center) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry)

(XBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the XBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot)

(Western blot analysis of XBP1 Antibody (Center) in Ramos cell line lysates (35ug/lane).XBP1 (arrow) was detected using the purified Pab.)

Related Product Information for anti-XBP1 antibody

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.

References

Navon, A., et al. FEBS Lett. 584(1):67-73(2010) Guan, D., et al. Mol. Carcinog. 49(1):68-74(2010) del Pozo, N., et al. Hum. Immunol. 70(11):950-952(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 118640873
• NCBI GeneID: 7494
• NCBI Accession #: NP_001073007.1; NP_005071.2
• NCBI GenBank Nucleotide #: NM_001079539.1
• UniProt Accession #: P17861; Q8WYK6; Q969P1; Q96BD7
• Molecular Weight: 28695
• NCBI Official Full Name: X-box-binding protein 1 isoform XBP1(S)
• NCBI Official Synonym Full Names: X-box binding protein 1
• NCBI Official Symbol: XBP1
• NCBI Official Synonym Symbols: XBP2; TREB5; XBP-1; TREB-5
• NCBI Protein Information: X-box-binding protein 1
• UniProt Protein Name: X-box-binding protein 1
• UniProt Gene Name: XBP1
• UniProt Synonym Gene Names: TREB-5
• UniProt Entry Name: XBP1_HUMAN