IHC (Immunohiostchemistry)
(Staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Rabbit XRCC1 Polyclonal Antibody | anti-XRCC1 antibody
Phospho-XRCC1 (Thr284) Antibody
Gene Names
XRCC1; RCC
Reactivity
HumanPredicted Reactivity: Horse (83%), Sheep (80%), Rabbit (83%), Dog (100%)
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
XRCC1, Antibody; Phospho-XRCC1 (Thr284) Antibody; DNA repair protein XRCC1; RCC; X ray repair complementing defective repair in chinese hamster; X ray Repair Complementing Defective Repair in Chinese Hamster Cells; X ray repair complementing defective repair in chinese hamster cells 1; X ray repair cross complementing 1; X ray repair cross complementing protein 1; X ray repair, complementing defective, repair in Chinese hamster; X-ray repair cross-complementing protein 1; XRCC 1; Xrcc1; XRCC1_HUMAN; anti-XRCC1 antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Horse (83%), Sheep (80%), Rabbit (83%), Dog (100%)
Predicted Reactivity: Horse (83%), Sheep (80%), Rabbit (83%), Dog (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-XRCC1 (Thr284) Antibody detects endogenous levels of XRCC1 only when phosphorylated at Thr284.
Tissue Specificity: Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
Tissue Specificity: Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level).
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-XRCC1 antibody
ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human XRCC1 around the phosphorylation site of Thr284.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF. Sumoylated.
Subunit Structure
Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF. Interacts with APEX1; the interaction is induced by SIRT1 and increases with the acetylated form of APEX1.
Subcellular Location
Nucleus.
Note: Moves from the nucleoli to the global nuclear chromatin upon DNA damage.
Note: Moves from the nucleoli to the global nuclear chromatin upon DNA damage.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IHC (Immunohiostchemistry)
(Staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IHC (Immunohiostchemistry)
(Staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot)
(Western blot analysis of XRCC1 (Phospho-Thr284) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
WB (Western Blot)
(Western blot analysis of XRCC1 (Phospho-Thr284) using HeLa whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-XRCC1 antibody
Involved in DNA single-strand break repair by mediating the assembly of DNA break repair protein complexes. Probably during DNA repair, negatively regulates ADP-ribose levels by modulating ADP-ribosyltransferase PARP1 activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
69,477 Da
NCBI Official Full Name
DNA repair protein XRCC1
NCBI Official Synonym Full Names
X-ray repair complementing defective repair in Chinese hamster cells 1
NCBI Official Symbol
XRCC1
NCBI Official Synonym Symbols
RCC
NCBI Protein Information
DNA repair protein XRCC1; X-ray repair cross-complementing protein 1; X-ray-repair, complementing defective, repair in Chinese hamster
UniProt Protein Name
DNA repair protein XRCC1
UniProt Gene Name
XRCC1
UniProt Entry Name
XRCC1_HUMAN
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Product Notes
The XRCC1 xrcc1 (Catalog #AAA329957) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-XRCC1 (Thr284) Antibody reacts with Human Predicted Reactivity: Horse (83%), Sheep (80%), Rabbit (83%), Dog (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's XRCC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the XRCC1 xrcc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XRCC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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