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WB (Western Blot) (WB Suggested Anti-YAP1 Antibody Titration: 1 ug/mlPositive Control: 721_B cell lysate)

Rabbit YAP1 Polyclonal Antibody | anti-YAP1 antibody

YAP1 antibody - C-terminal region

Gene Names
YAP1; YAP; YKI; COB1; YAP2; YAP65
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
YAP1; Polyclonal Antibody; YAP1 antibody - C-terminal region; anti-YAP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Tested Reactivity: Human
Predicted Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Clonality
Polyclonal
Purity/Purification
Affinity Purified
Form/Format
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration
0.5 mg/mL (varies by lot)
Sequence
QLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDS
Applicable Applications for anti-YAP1 antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC Information: Human Prostate (formalin-fixed, paraffin-embedded) stained with YAP1 antibody AAA23538 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
IHC Information: Human Prostate (formalin-fixed, paraffin-embedded) stained with YAP1 antibody AAA23538 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Protein Size (# AA)
504 amino acids
Protein Interactions
ABL1; TP73; RUNX1; AMOT; UBC; FBXW7; ARFIP1; RTF1; PAPOLA; PRMT3; TOM1; TSNAX; STAT1; MAP2K1; NF2; WBP2; RASSF8; YWHAQ; INADL; RAPGEF2; TJP2; SLC9A3R2; PDLIM7; LATS1; MPDZ; RAE1; ARID1A; YWHAZ; YWHAH; YWHAG; YWHAE; YWHAB; TP53BP2; TEAD3; TEAD1; SMARCD1; D
Blocking Peptide
For anti-YAP1 antibody
Immunogen
The immunogen is a synthetic peptide directed towards the C terminal region of human YAP1
Protein Name
Yorkie homolog
Replacement Item
This antibody may replace item sc-124676 from Santa Cruz Biotechnology.
Predicted Homology
Cow: 100%; Dog: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 100%; Sheep: 100%; Zebrafish: 100%
Preparation and Storage
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(WB Suggested Anti-YAP1 Antibody Titration: 1 ug/mlPositive Control: 721_B cell lysate)

WB (Western Blot) (WB Suggested Anti-YAP1 Antibody Titration: 1 ug/mlPositive Control: 721_B cell lysate)

WB (Western Blot)

(Host: RabbitTarget Name: YAP1Sample Type: Human Fetal Lung cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%)

WB (Western Blot) (Host: RabbitTarget Name: YAP1Sample Type: Human Fetal Lung cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%)

WB (Western Blot)

(Host: RabbitTarget Name: YAP1Sample Type: Fetal Liver lysatesAntibody Dilution: 3ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: YAP1Sample Type: Fetal Liver lysatesAntibody Dilution: 3ug/ml)

WB (Western Blot)

(Host: RabbitTarget Name: YAP1Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: YAP1Sample Tissue: Human Fetal LungAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry)

(Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-YAP1 antibody)

IHC (Immunohistochemistry) (Immunohistochemistry with Human Prostate lysate tissue at an antibody concentration of 5.0ug/ml using anti-YAP1 antibody)

IHC (Immunohistochemistry)

(Human Prostate)

IHC (Immunohistochemistry) (Human Prostate)
Related Product Information for anti-YAP1 antibody
This is a rabbit polyclonal antibody against YAP1. It was validated on Western Blot using a cell lysate as a positive control.

Target Description: YAP1 is the human ortholog of chicken YAP protein which binds to the SH3 domain of the Yes proto-oncogene product. YAP1 contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54kDa
NCBI Official Full Name
transcriptional coactivator YAP1 isoform 1
NCBI Official Synonym Full Names
Yes associated protein 1
NCBI Official Symbol
YAP1
NCBI Official Synonym Symbols
YAP; YKI; COB1; YAP2; YAP65
NCBI Protein Information
transcriptional coactivator YAP1
UniProt Protein Name
Yorkie homolog
UniProt Gene Name
YAP1
UniProt Synonym Gene Names
YAP65; YAP65
UniProt Entry Name
YAP1_HUMAN

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Product Notes

The YAP1 yap1 (Catalog #AAA23538) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The YAP1 antibody - C-terminal region reacts with Tested Reactivity: Human Predicted Reactivity: Cow, Dog, Horse, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's YAP1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC Information: Human Prostate (formalin-fixed, paraffin-embedded) stained with YAP1 antibody AAA23538 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. IHC Information: Human Prostate (formalin-fixed, paraffin-embedded) stained with YAP1 antibody AAA23538 followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Researchers should empirically determine the suitability of the YAP1 yap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: QLPTLEQDGG TQNPVSSPGM SQELRTMTTN SSDPFLNSGT YHSRDESTDS. It is sometimes possible for the material contained within the vial of "YAP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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