Rabbit anti-Human ZAP70 Polyclonal Antibody | anti-ZAP70 antibody

Rabbit anti-Phospho ZAP70 (Y319) Antibody, Affinity Purified

Cat. Num. AAA211718

• Gene Names: ZAP70; SRK; STD; TZK; STCD; ZAP-70
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation
• Purity: Antigen Affinity Purified
• Synonyms: ZAP70, Antibody; Rabbit anti-Phospho ZAP70 (Y319) Antibody, Affinity Purified; ZAP70, Phospho (Y319); ZAP-70; syk-related tyrosine kinase; zeta chain of T cell receptor associated protein kinase 70kDa; TZK; tyrosine-protein kinase ZAP-70; zeta-chain associated protein kinase, 70kD; STCD; SRK; IMD48; ADMIO2; 70 kDa zeta-chain associated protein; 70 kDa zeta-associated protein; zeta-chain (TCR) associated protein kinase 70kDa; STD; Tyrosine-protein kinase ZAP-70; zeta chain of T cell receptor associated protein kinase 70; anti-ZAP70 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000ug/ml (varies by lot)

• Applicable Applications for anti-ZAP70 antibody: FCM/FACS (Flow Cytometry), IP (Immunoprecipitation)
• Predicted Reactivity: Mouse
• Immunogen: Surrounding tyrosine 319
• Conjugation: Unconjugated
• Preparation and Storage: Store at 2-8 degree C; Shelf Life: 1 year from date of receipt

IP (Immunoprecipitation)

(Detection of Phosphorylation of human ZAP70 on Y319 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: ZAP70 antibody was used at 3 ug/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated. For blotting immunoprecipitated Phospho ZAP70, Phospho ZAP70 (Y319) antibody AAA211718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IP (Immunoprecipitation)

(Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Phospho ZAP70 antibodies AAA211718 (phosphorylation at Y319), (phosphorylation at Y493), and (phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, ZAP70 antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 min.)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10^6 cells stained with 1.0 ug/ml Phospho Zap70 (Y319) antibody AAA211718 in a 150 ul volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), anti-phospho Zap70 (Y319) in H2O2 treated (blue).)

Related Product Information for anti-ZAP70 antibody

ZAP-70 (zeta associated protein 70) is a tyrosine kinase of the Src family of tyrosine kinases. It was initially identified as a partner of the zeta chain of the CD3 complex associated to the T-cell receptor on T-lymphocytes. ZAP-70 shares homology with spleen-tyrosine kinase (Syk), a kinase involved in B-cell activation. Both ZAP-70 and Syk have been shown to be crucial to B- and T-lymphocyte development. In T-cells ZAP-70 functions to transduce the signaling cascade activated by T-cell receptor engagement and activation.

Product Categories/Family for anti-ZAP70 antibody

Primary Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 31455611
• NCBI GeneID: 7535
• NCBI Accession #: NP_001070.2
• NCBI GenBank Nucleotide #: NM_001079.3
• UniProt Accession #: P43403; Q6PIA4; Q8IXD6; Q9UBS6; A6NFP4
• Molecular Weight: 69,872 Da
• NCBI Official Full Name: tyrosine-protein kinase ZAP-70 isoform 1
• NCBI Official Synonym Full Names: zeta-chain (TCR) associated protein kinase 70kDa
• NCBI Official Symbol: ZAP70
• NCBI Official Synonym Symbols: SRK; STD; TZK; STCD; ZAP-70
• NCBI Protein Information: tyrosine-protein kinase ZAP-70; syk-related tyrosine kinase; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; zeta-chain associated protein kinase, 70kD
• UniProt Protein Name: Tyrosine-protein kinase ZAP-70
• UniProt Gene Name: ZAP70
• UniProt Synonym Gene Names: SRK
• UniProt Entry Name: ZAP70_HUMAN